Journal article
Respiratory syncytial virus nonstructural protein 2 specifically inhibits type I interferon signal transduction
Virology (New York, N.Y.), Vol.344(2), pp.328-339
2006
DOI: 10.1016/j.virol.2005.09.009
PMID: 16216295
Abstract
Human respiratory syncytial virus (RSV) inhibits type I interferon-induced gene expression by decreasing expression of signal transducer and activator of transcription (Stat)2. To identify the RSV protein that mediates effects on Stat2, airway epithelial cells were infected with vaccinia virus vectors that express single RSV proteins. Expression of RSV nonstructural (NS)2 protein alone was sufficient to decrease Stat2 levels. Furthermore, decreasing RSV NS2 levels using RNA interference in respiratory epithelial cells inhibited the RSV-mediated decrease in Stat2 expression. Airway epithelial cells were also infected with equivalent inoculums of RSV without or with single gene deletions of NS1 or NS2. RSV infection without NS2 expression did not result in decreased Stat2 levels or loss of type I interferon-dependent signaling, indicating that NS2 expression is necessary for RSV effects on Stat2. Taken together, our results indicate that NS2 regulates Stat2 levels during RSV infection, thereby modulating viral effects on interferon-dependent gene expression.
Details
- Title: Subtitle
- Respiratory syncytial virus nonstructural protein 2 specifically inhibits type I interferon signal transduction
- Creators
- Murali Ramaswamy - Department of Internal Medicine, University of Iowa Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA 52242, USALei Shi - Department of Internal Medicine, University of Iowa Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA 52242, USASteven M Varga - Department of Microbiology, University of Iowa Carver College of Medicine, 3-532 Bowen Sciences Building, Iowa City, IA 52242, USASailen Barik - Department of Biochemistry and Molecular Biology, University of South Alabama College of Medicine, 307 University Blvd., Room MSB 2370, Mobile, AL 36688, USAMark A Behlke - Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USADwight C Look - Department of Internal Medicine, University of Iowa Carver College of Medicine, 200 Hawkins Drive, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Virology (New York, N.Y.), Vol.344(2), pp.328-339
- DOI
- 10.1016/j.virol.2005.09.009
- PMID
- 16216295
- NLM abbreviation
- Virology
- ISSN
- 0042-6822
- eISSN
- 1096-0341
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2006
- Academic Unit
- Graduate College Admin and Gen; Microbiology and Immunology; Pathology
- Record Identifier
- 9984083270902771
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