Journal article
Response of Human Embryonic Stem Cell–Derived Mesenchymal Stem Cells to Osteogenic Factors and Architectures of Materials During In Vitro Osteogenesis
Tissue engineering. Part A, Vol.16(11), pp.3507-3514
08/10/2010
DOI: 10.1089/ten.tea.2010.0097
PMCID: PMC2965192
PMID: 20594153
Abstract
One of the major challenges to the application of human embryonic stem cells (hESCs) to the repair of defective tissues is the directed differentiation of cells into specific lineages to avoid the formation of inferior heterogeneous tissues. To accomplish this goal, the lineage-specific stem cell population needs to be isolated and optimal differentiation conditions need to be defined. In this study, homogenous hESC-derived mesenchymal stem cells (hESC-MSCs) were generated and used to construct bone tissue. The effect of osteogenic factors, including dexamethasone (Dex) and bone morphogenetic protein-7 (BMP-7), on the osteogenesis of hESC-MSCs was investigated. It was found that BMP-7 itself had little effect on the
in vitro
osteogenic differentiation of hESC-MSCs; however, there was a synergic effect between BMP-7 and Dex in promoting osteogenesis. The effect of osteoconductive nanofibrous polylactic acid material on osteogenesis of hESC-MSCs was also investigated. It was found that the nanofibrous matrix architecture promoted alkaline phosphatase activity and calcium deposition of cells cultured under osteogenic conditions. Based on these findings, the hESC-MSCs were cultured on three-dimensional nanofibrous scaffolds in combination with Dex and BMP-7 stimulation
in vitro
to generate bone-like tissues. After 6 weeks of culture, highly mineralized tissues developed with specific bone marker genes expressed. These data illustrate the promise of hESC-MSCs for bone regeneration under optimal conditions.
Details
- Title: Subtitle
- Response of Human Embryonic Stem Cell–Derived Mesenchymal Stem Cells to Osteogenic Factors and Architectures of Materials During In Vitro Osteogenesis
- Creators
- Jiang Hu - University of MichiganLaura A. Smith - The University of MichiganKai Feng - University of MichiganXiaohua Liu - University of MichiganHongli Sun - University of MichiganPeter X. Ma - Institute of Macromolecular Chemistry
- Resource Type
- Journal article
- Publication Details
- Tissue engineering. Part A, Vol.16(11), pp.3507-3514
- DOI
- 10.1089/ten.tea.2010.0097
- PMID
- 20594153
- PMCID
- PMC2965192
- NLM abbreviation
- Tissue Eng Part A
- ISSN
- 1937-3341
- eISSN
- 1937-335X
- Publisher
- Mary Ann Liebert, Inc
- Language
- English
- Date published
- 08/10/2010
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery
- Record Identifier
- 9984367755702771
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