Restricted and coordinated expression of beta3-integrin and bone sialoprotein during cultured osteoblast differentiation

G B Schneider; S W Whitson; L F Cooper

doi:10.1016/s8756-3282(99)00007-1

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Restricted and coordinated expression of beta3-integrin and bone sialoprotein during cultured osteoblast differentiation

Journal article

Peer reviewed

Restricted and coordinated expression of beta3-integrin and bone sialoprotein during cultured osteoblast differentiation

G B Schneider, S W Whitson and L F Cooper

Bone (New York, N.Y.), Vol.24(4), pp.321-327

04/1999

DOI: 10.1016/s8756-3282(99)00007-1

PMID: 10221544

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Abstract

In this study, the expression of beta3-integrin was examined in relationship to the restricted expression of bone sialoprotein (BSP). Immunohistochemical analysis indicated that the alpha(v)beta3-integrin was coincident and proximal to BSP expression in the fetal mandible bovine osteoblast culture model. Alpha(v)beta3-integrin expression was expressed predominantly in a region proximal to, but not including, the substrate adherent cells. In comparison, the alpha5beta1-integrin was expressed in a generalized pattern throughout the culture layers in a coordinated fashion to fibronectin. The temporal expression of beta1- and beta3-integrin was evaluated using RT-PCR and southern blot analysis. Unlike the generalized expression of beta1-integrin, beta3-integrin was restricted to days 3 and 5 of the culture period. The previous demonstration of similar restriction of BSP expression and the present colocalization of BSP suggests the potential coordinated expression of a specific extracellular matrix ligand with a select integrin. Beta3-integrin/BSP adhesion-mediated signaling may play a significant role in the process of osteoblast morphodifferentiation.

Immunohistochemistry

Blotting, Southern

Fibronectins - analysis

Antigens, CD - biosynthesis

Integrin-Binding Sialoprotein

Cells, Cultured

Integrin beta3

Reverse Transcriptase Polymerase Chain Reaction

RNA, Messenger - biosynthesis

Sialoglycoproteins - biosynthesis

Receptors, Vitronectin - analysis

Animals

Cattle

Cell Adhesion - physiology

Platelet Membrane Glycoproteins - biosynthesis

Osteoblasts - cytology

Osteoblasts - metabolism

Cell Differentiation - physiology

Details

Title: Subtitle: Restricted and coordinated expression of beta3-integrin and bone sialoprotein during cultured osteoblast differentiation
Creators: G B Schneider - Dental Research Center and Department of Prosthodontics, The University of North Carolina School of Dentistry, Chapel Hill 27599-7450, USA
S W Whitson
L F Cooper
Resource Type: Journal article
Publication Details: Bone (New York, N.Y.), Vol.24(4), pp.321-327
DOI: 10.1016/s8756-3282(99)00007-1
PMID: 10221544
NLM abbreviation: Bone
ISSN: 8756-3282
eISSN: 1873-2763
Publisher: United States
Language: English
Date published: 04/1999
Academic Unit: Dentistry Administration; Prosthodontics
Record Identifier: 9984065805902771

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