Journal article
Restriction enzyme fragment length polymorphisms of amplified herpes simplex virus type-1 DNA provide epidemiologic information
Diagnostic microbiology and infectious disease, Vol.17(2), pp.129-133
1993
DOI: 10.1016/0732-8893(93)90023-Z
PMID: 7902227
Abstract
Human herpes simplex type 1 (HSV-1) DNA of isolates from patients in a large teaching hospital was amplified by the polymerase chain reaction (PCR). The PCR products targeted ∼2100 nt regions of relatively low G + C content. Comparison of restriction enzyme digests of amplified DNA showed variation useful for strain differentiation. Twelve nonrelated HSV-1 were differentiated from one another. In contrast, specimens epidemiologically related in an outbreak were indistinguishable from each other. Restriction endonuclease analysis of amplified HSV-1 sequences appears to be useful for molecular epidemiology and laboratory quality control to detect possible contamination by PCR products.
Details
- Title: Subtitle
- Restriction enzyme fragment length polymorphisms of amplified herpes simplex virus type-1 DNA provide epidemiologic information
- Creators
- Thomas H HaugenBeth AldenSandra MattheyDonald Nicholson
- Resource Type
- Journal article
- Publication Details
- Diagnostic microbiology and infectious disease, Vol.17(2), pp.129-133
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0732-8893(93)90023-Z
- PMID
- 7902227
- ISSN
- 0732-8893
- eISSN
- 1879-0070
- Language
- English
- Date published
- 1993
- Academic Unit
- Pathology
- Record Identifier
- 9984047616902771
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