Journal article
Retinal ganglion cells survival in a glaucoma model by GDNF/Vit E PLGA microspheres prepared according to a novel microencapsulation procedure
Journal of Controlled Release, Vol.156(1), pp.92-100
2011
DOI: 10.1016/j.jconrel.2011.06.023
PMID: 21704662
Abstract
The present experimental work describes the use of a novel protein encapsulation method to achieve protection of the biological factor during the microencapsulation procedure. With this aim, the protein is included in poly(lactic-co-glycolic acid) (PLGA) microspheres without any preliminary manipulation, in contrast to the traditional S/O/W (solid-in-oil-in-water) method where the bioactive substance is first dissolved and then freeze-dried in the presence of lyoprotectors. Furthermore, the presented technique involves the use of an oily additive, vitamin E (Vit E), useful from a technological point of view, by promoting additional protein protection and also from a pharmacological point of view, because of its antioxidant and antiproliferative properties. Application of this microencapsulation technique has been performed for GDNF (glial cell line-derived neurotrophic factor) designed for the treatment of optic nerve degenerative diseases, such as glaucoma, the second leading cause of blindness in the western world. The protein was released in vitro in its bioactive form for more than three months, demonstrated by the survival of their potential target cells (photoreceptors and retinal ganglion cells (RGC)). Moreover, the intravitreal injection of GDNF/Vit E PLGA microspheres in an experimental animal model of glaucoma significantly increased RGC survival compared with GDNF, Vit E or blank microspheres (p < 0.01). This effect was present for at least eleven weeks, which suggests that the formulation prepared may be clinically useful as a neuroprotective tool in the treatment of glaucomatous optic neuropathy. [Display omitted]
Details
- Title: Subtitle
- Retinal ganglion cells survival in a glaucoma model by GDNF/Vit E PLGA microspheres prepared according to a novel microencapsulation procedure
- Creators
- Patricia Checa-Casalengua - Dep. of Pharmacy and Pharmaceutical Technology, School of Pharmacy, Complutense University, Avda. Complutense s/n, Madrid 28040, SpainCaihui Jiang - Schepens Eye Research Institute, Dep. of Ophthalmology, Harvard Medical School, 20 Staniford Street, Boston, MA 02114, USAIrene Bravo-Osuna - Dep. of Pharmacy and Pharmaceutical Technology, School of Pharmacy, Complutense University, Avda. Complutense s/n, Madrid 28040, SpainBudd A Tucker - Institute for Vision Research, Department of Ophthalmology, Carver College of Medicine, University of Iowa, 200 CMAB, Iowa City, IA 52242, USAIrene T Molina-Martínez - Dep. of Pharmacy and Pharmaceutical Technology, School of Pharmacy, Complutense University, Avda. Complutense s/n, Madrid 28040, SpainMichael J Young - Schepens Eye Research Institute, Dep. of Ophthalmology, Harvard Medical School, 20 Staniford Street, Boston, MA 02114, USARocío Herrero-Vanrell - Dep. of Pharmacy and Pharmaceutical Technology, School of Pharmacy, Complutense University, Avda. Complutense s/n, Madrid 28040, Spain
- Resource Type
- Journal article
- Publication Details
- Journal of Controlled Release, Vol.156(1), pp.92-100
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.jconrel.2011.06.023
- PMID
- 21704662
- ISSN
- 0168-3659
- eISSN
- 1873-4995
- Grant note
- DOI: 10.13039/501100003176, name: Ministerio de Educación, Cultura y Deporte; DOI: 10.13039/501100004837, name: Ministerio de Ciencia e Innovación, award: MAT-2010-18242, PSE-300-100, UCM 920415 GR 35/10-A
- Language
- English
- Date published
- 2011
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9983979978502771
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