Journal article
Retinal synthesis and deposition of complement components induced by ocular hypertension
Experimental Eye Research, Vol.83(3), pp.620-628
2006
DOI: 10.1016/j.exer.2006.03.002
PMID: 16677633
Abstract
Inappropriate activity of the complement cascade contributes to the pathophysiology of several neurodegenerative conditions. This study sought to determine if components of the complement cascade are synthesized in the retina following the development of ocular hypertension (OHT) and if complement accumulates in association with retinal ganglion cells. Toward this goal the gene expression levels of complement components 1qb ( C1qb) and 3 ( C3) were determined in the retina by quantitative polymerase chain reaction in human eyes with elevated intraocular pressure (IOP) and healthy retinal tissue as well as in a rat model of OHT induced by laser cauterization of the trabecular meshwork and episcleral veins. Immunohistochemical methods were employed to determine the sites of complement deposition in the retina and optic nerve head. Our data demonstrate that transcript levels for C1q and C3 are significantly elevated in retinae subjected to OHT, both in the animal model as well as in human eyes. Immunohistochemical analyses indicate that C1q and C3 accumulate specifically in the retinal ganglion cell layer and the nerve fiber layer. In addition, we demonstrate that the terminal complement complex, or membrane attack complex, is formed both in the human and rat model as a consequence of OHT. Complement activation, particularly formation of membrane attack complexes, has the potential to exacerbate ganglion cell death through bystander lysis or glial cell activation. The results show that complement activation occurs in the retina that has been subjected to elevated IOP, and may have implications in pathophysiology of glaucoma.
Details
- Title: Subtitle
- Retinal synthesis and deposition of complement components induced by ocular hypertension
- Creators
- Markus H Kuehn - Department of Ophthalmology and Visual Sciences, University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, USAChan Y Kim - Department of Ophthalmology and Visual Sciences, University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, USAJelena Ostojic - Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USAMicheal Bellin - Department of Veterinary Clinical Sciences, Iowa State University, Ames, IA, USAWallace L.M Alward - Department of Ophthalmology and Visual Sciences, University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, USAEdwin M Stone - Department of Ophthalmology and Visual Sciences, University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, USADonald S Sakaguchi - Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, USASinisa D Grozdanic - Department of Veterinary Clinical Sciences, Iowa State University, Ames, IA, USAYoung H Kwon - Department of Ophthalmology and Visual Sciences, University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Experimental Eye Research, Vol.83(3), pp.620-628
- DOI
- 10.1016/j.exer.2006.03.002
- PMID
- 16677633
- NLM abbreviation
- Exp Eye Res
- ISSN
- 0014-4835
- eISSN
- 1096-0007
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 2006
- Academic Unit
- Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
- Record Identifier
- 9983980281602771
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