Journal article
Retinoic Acid Signaling in B Cells Is Required for the Generation of an Effective T-Independent Immune Response
Frontiers in immunology, Vol.7, pp.643-643
2016
DOI: 10.3389/fimmu.2016.00643
PMCID: PMC5179524
PMID: 28066447
Abstract
Retinoic acid (RA) plays an important role in the balance of inflammation and tolerance in T cells. Furthermore, it has been demonstrated that RA facilitates IgA isotype switching in B cells
in vivo
. However, it is unclear whether RA has a direct effect on T-independent B cell responses
in vivo
. To address this question, we generated a mouse model where RA signaling is specifically silenced in the B cell lineage. This was achieved through the overexpression of a dominant negative receptor α for RA (dnRARα) in the B cell lineage. In this model, we found a dramatic reduction in marginal zone (MZ) B cells and accumulation of transitional 2 B cells in the spleen. We also observed a reduction in B1 B cells in the peritoneum with a defect in the T-independent B cell response against 2,4,6-trinitrophenyl. This was not a result of inhibited development of B cells in the bone marrow, but likely the result of both defective expression of S1P
1
in MZ B cells and a defect in the development of MZ and B1 B cells. This suggests that RARα expression in B cells is important for B cell frequency in the MZ and peritoneum, which is crucial for the generation of T-independent humoral responses.
Details
- Title: Subtitle
- Retinoic Acid Signaling in B Cells Is Required for the Generation of an Effective T-Independent Immune Response
- Creators
- Ellen Marks - Department of Mucosal Immunology, Division of Transplantation Immunology & Mucosal Biology, Guy’s Hospital, King’s College LondonCarla Ortiz - Department of Immune Regulation and Intervention, Division of Transplantation Immunology & Mucosal Biology, Guy’s Hospital, King’s College LondonEirini Pantazi - Department of Immune Regulation and Intervention, Division of Transplantation Immunology & Mucosal Biology, Guy’s Hospital, King’s College LondonCharlotte S Bailey - Department of Immune Regulation and Intervention, Division of Transplantation Immunology & Mucosal Biology, Guy’s Hospital, King’s College LondonGraham M Lord - Department of Mucosal Immunology, Division of Transplantation Immunology & Mucosal Biology, Guy’s Hospital, King’s College LondonThomas J Waldschmidt - Interdisciplinary Graduate Program in Immunology, Carver College of Medicine, The University of IowaRandolph J Noelle - Department of Immune Regulation and Intervention, Division of Transplantation Immunology & Mucosal Biology, Guy’s Hospital, King’s College LondonRaul Elgueta - Department of Immune Regulation and Intervention, Division of Transplantation Immunology & Mucosal Biology, Guy’s Hospital, King’s College London
- Resource Type
- Journal article
- Publication Details
- Frontiers in immunology, Vol.7, pp.643-643
- DOI
- 10.3389/fimmu.2016.00643
- PMID
- 28066447
- PMCID
- PMC5179524
- NLM abbreviation
- Front Immunol
- ISSN
- 1664-3224
- eISSN
- 1664-3224
- Publisher
- Frontiers Media S.A
- Grant note
- MR/J006742/1 / Medical Research Council 091823/z/10/z / Wellcome Trust National Institute for Health Research
- Language
- English
- Date published
- 2016
- Academic Unit
- Pathology
- Record Identifier
- 9984046813402771
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