Journal article
Retinold-related orphan receptor gamma controls immunoglobulin production and Th1/Th2 cytokine mance in the adaptive immune response to allergen
The Journal of immunology (1950), Vol.178(5), pp.3208-3218
03/01/2007
DOI: 10.4049/jimmunol.178.5.3208
PMID: 17312169
Abstract
The retinoid-related orphan receptors (ROR) comprise a distinct subfamily of nuclear receptors with the capacity to act as both repressors and activators of transcription. ROR gamma, the most recently identified member of the ROR family, has been shown to be important for the development of normal lymphocyte compartments as well as organogenesis of some lymphoid organs. In this report, we examine the capacity of ROR gamma-deficient mice to develop an adaptive immune response to Ag using OVA-induced inflammation in mice as a model for allergic airway disease. In sham-treated mice lacking ROR gamma, low-grade pulmonary inflammation was observed and characterized by the perivascular accumulation of B and T lymphocytes, increased numbers of inflammatory cells in the lung lavage fluid, and polyclonal Ig activation. Following sensitization and challenge, the capacity of these animals to develop the allergic phenotype was severely impaired as evidenced by attenuated eosinophilic pulmonary inflammation, reduced numbers of CD4(+) lymphocytes, and lower Th2 cytokines/chemokine protein and mRNA expression in the lungs. IFN-gamma and IL-10 production was markedly greater in splenocytes from ROR gamma-deficient mice following in vitro restimulation with OVA compared with wild-type splenocytes, and a shift toward a Th1 immune response was observed in sensitized/challenged ROR gamma-deficient animals in vivo. These data reveal a critical role for ROR gamma in the regulation of Ig production and Th1/Th2 balance in adaptive immunity.
Details
- Title: Subtitle
- Retinold-related orphan receptor gamma controls immunoglobulin production and Th1/Th2 cytokine mance in the adaptive immune response to allergen
- Creators
- Stephen L. Tilley - *Department of Medicine, Division of Pulmonary and Critical Care Medicine, andMaisa Jaradat - Laboratory of BioChemistryCliona Stapleton - Laboratory of BioChemistryDarlene Dixon - National Institute of Environmental Health SciencesXiaoyang Hua - *Department of Medicine, Division of Pulmonary and Critical Care Medicine, andChristopher J. Erikson - *Department of Medicine, Division of Pulmonary and Critical Care Medicine, andJoshua G. McCaskill - *Department of Medicine, Division of Pulmonary and Critical Care Medicine, andKelly D. Chason - *Department of Medicine, Division of Pulmonary and Critical Care Medicine, andGrace Liao - Laboratory of BioChemistryLeigh Jania - University of North Carolina at Chapel HillBeverly H. Koller - University of North Carolina at Chapel HillAnton M. Jetten - Laboratory of BioChemistry
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.178(5), pp.3208-3218
- DOI
- 10.4049/jimmunol.178.5.3208
- PMID
- 17312169
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- Amer Assoc Immunologists
- Number of pages
- 11
- Grant note
- Z01ES021196 / NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS) R01HL071802 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) Intramural NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA HL 071802 / NHLBI NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI)
- Language
- English
- Date published
- 03/01/2007
- Academic Unit
- Otolaryngology
- Record Identifier
- 9984312248202771
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