Retrovirus vector-targeted inducible expression of human β-interferon gene to B-cells

John F Engelhardt; Merrill J Kellum; Fatina Bisat; Paula M Pitha

doi:10.1016/0042-6822(90)90339-S

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Retrovirus vector-targeted inducible expression of human β-interferon gene to B-cells

Journal article

Open access

Peer reviewed

Retrovirus vector-targeted inducible expression of human β-interferon gene to B-cells

John F Engelhardt, Merrill J Kellum, Fatina Bisat and Paula M Pitha

Virology (New York, N.Y.), Vol.178(2), pp.419-428

1990

DOI: 10.1016/0042-6822(90)90339-S

PMID: 2171189

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Abstract

We have introduced the human β-interferon gene with its promoter region into murine B-cell and fibroblast cell lines via a Moloney murine leukemia virus (M-MuLV) vector and have studied the inducible expression of the β-interferon gene as a function of the various retroviral vector designs. By deleting the enhancer within the 3′ viral long terminal repeat (LTR), inserting the human β-interferon gene, and varying placement of the immunoglobulin heavy chain enhancer, we were able to construct vectors which yielded proviruses with various cell type-specific regulation. One of the vectors (pT154) led to a greater than 21-fold increase in β-interferon protein synthesis after viral infection in the two B-cell lines analyzed, while no inducibility was seen in the fibroblast cells. The data show that inducible β-interferon expression within a MuLV vector was highly dependent on the absence of the viral enhancer region in the long terminal repeat and the orientation of the β-interferon gene within the proviral transcriptional unit; the insertion of the immunoglobulin enhancer elevated both constitutive and (or) inducible expression of β-interferon in B-cells but inhibited constitutive expression of this gene in fibroblasts.

Details

Title: Subtitle: Retrovirus vector-targeted inducible expression of human β-interferon gene to B-cells
Creators: John F Engelhardt - Johns Hopkins Medicine
Merrill J Kellum
Fatina Bisat
Paula M Pitha - Johns Hopkins Medicine
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.178(2), pp.419-428
DOI: 10.1016/0042-6822(90)90339-S
PMID: 2171189
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: Elsevier Inc
Language: English
Date published: 1990
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
Record Identifier: 9984284335002771

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