Journal article
Reversibility of PRKAG2 Glycogen-Storage Cardiomyopathy and Electrophysiological Manifestations
Circulation (New York, N.Y.), Vol.117(2), pp.144-154
2008
DOI: 10.1161/CIRCULATIONAHA.107.726752
PMCID: PMC2957811
PMID: 18158359
Abstract
Background: PRKAG2 mutations cause glycogen-storage cardiomyopathy, ventricular preexcitation, and conduction system degeneration. A genetic approach that utilizes a binary inducible transgenic system was used to investigate the disease mechanism and to assess preventability and reversibility of disease features in a mouse model of glycogen-storage cardiomyopathy.
Methods and results: Transgenic (Tg) mice expressing a human N488I PRKAG2 cDNA under control of the tetracycline-repressible alpha-myosin heavy chain promoter underwent echocardiography, ECG, and in vivo electrophysiology studies. Transgene suppression by tetracycline administration caused a reduction in cardiac glycogen content and was initiated either prenatally (Tg(OFF(E-8 weeks))) or at different time points during life (Tg(OFF(4-16 weeks)), Tg(OFF(8-20 weeks)), and Tg(OFF(>20 weeks))). One group never received tetracycline, expressing transgene throughout life (Tg(ON)). Tg(ON) mice developed cardiac hypertrophy followed by dilatation, ventricular preexcitation involving multiple accessory pathways, and conduction system disease, including sinus and atrioventricular node dysfunction.
Conclusions: Using an externally modifiable transgenic system, cardiomyopathy, cardiac dysfunction, and electrophysiological disorders were demonstrated to be reversible processes in PRKAG2 disease. Transgene suppression during early postnatal development prevented the development of accessory electrical pathways but not cardiomyopathy or conduction system degeneration. Taken together, these data provide insight into mechanisms of cardiac PRKAG2 disease and suggest that glycogen-storage cardiomyopathy can be modulated by lowering glycogen content in the heart.
Details
- Title: Subtitle
- Reversibility of PRKAG2 Glycogen-Storage Cardiomyopathy and Electrophysiological Manifestations
- Creators
- Cordula M WOLF - Department of Cardiology, Children's Hospital Boston, United StatesMichael ARAD - Sheba Medical Center Tel Aviv University, IsraelChristine E SEIDMAN - Department of Genetics ,arvard Medical School, Boston, Mass, United StatesCharles I BERUL - Department of Cardiology, Children's Hospital Boston, United StatesFerhaan AHMAD - Cardiovascular Institute and Department of Medicine and Human Genetics , University of Pittsburgh, Pittsburgh, Pa, United StatesAtsushi SANBE - Molecular Cardiovascular Biology , Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, United StatesScott A BERNSTEIN - Department of Cardiology, New York University School of Medicine, New York, NY, United StatesOkan TOKA - Department of Cardiology, Friedrich-Alexander University, Erlangen, GermanyTetsuo KONNO - Department of Genetics ,arvard Medical School, Boston, Mass, United StatesGregory MORLEY - Department of Cardiology, New York University School of Medicine, New York, NY, United StatesJeffrey ROBBINS - Molecular Cardiovascular Biology , Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, United StatesJ. G SEIDMAN - Department of Genetics ,arvard Medical School, Boston, Mass, United States
- Resource Type
- Journal article
- Publication Details
- Circulation (New York, N.Y.), Vol.117(2), pp.144-154
- DOI
- 10.1161/CIRCULATIONAHA.107.726752
- PMID
- 18158359
- PMCID
- PMC2957811
- NLM abbreviation
- Circulation
- ISSN
- 0009-7322
- eISSN
- 1524-4539
- Publisher
- Lippincott Williams & Wilkins; Hagerstown, MD
- Language
- English
- Date published
- 2008
- Academic Unit
- Radiology; Molecular Physiology and Biophysics; Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984025598302771
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