Reversion of a gypsy-induced mutation at the yellow (y) locus of Drosophila melanogaster is associated with the insertion of a newly defined transposable element

Pamela K Geyer; M M Green; Victor G Corces

doi:10.1073/pnas.85.11.3938

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Reversion of a gypsy-induced mutation at the yellow (y) locus of Drosophila melanogaster is associated with the insertion of a newly defined transposable element

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Reversion of a gypsy-induced mutation at the yellow (y) locus of Drosophila melanogaster is associated with the insertion of a newly defined transposable element

Pamela K Geyer, M M Green and Victor G Corces

Proceedings of the National Academy of Sciences - PNAS, Vol.85(11), pp.3938-3942

06/1988

DOI: 10.1073/pnas.85.11.3938

PMCID: PMC280335

PMID: 2836866

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Abstract

To understand the molecular basis of the phenotype of gypsy-induced mutations, we have analyzed the structure of phenotypic revertants of the y2 allele, which is caused by the insertion of the gypsy element into the 5' region of the yellow (y) locus. Seven spontaneous revertants examined fall into two different classes. Three of these revertants arose by homologous recombination between the two gypsy long terminal repeats (LTRs), leaving behind a solo LTR. Four additional revertants contain an intact 3' LTR and half of the 5' LTR, but the central portion of gypsy has been replaced by a different 6.5-kilobase transposable element that contains a poly(A) tail. These results suggest that the mutagenic effect of the gypsy element is not due to its insertion into sequences necessary for transcription or to the distancing between the yellow promoter and remote regulatory sequences but is a consequence of idiosyncratic properties of the element itself.

Details

Title: Subtitle: Reversion of a gypsy-induced mutation at the yellow (y) locus of Drosophila melanogaster is associated with the insertion of a newly defined transposable element
Creators: Pamela K Geyer - Department of Biology, Johns Hopkins University, Baltimore, MD 21218
M M Green - Department of Biology, Johns Hopkins University, Baltimore, MD 21218
Victor G Corces - Department of Biology, Johns Hopkins University, Baltimore, MD 21218
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.85(11), pp.3938-3942
DOI: 10.1073/pnas.85.11.3938
PMID: 2836866
PMCID: PMC280335
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 06/1988
Academic Unit: Obstetrics and Gynecology; Biochemistry and Molecular Biology
Record Identifier: 9984025254602771

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