Journal article
Ribosome Biogenesis Modulates Ty1 Copy Number Control in Saccharomyces cerevisiae
Genetics (Austin), Vol.207(4), pp.1441-1456
12/01/2017
DOI: 10.1534/genetics.117.300388
PMCID: PMC5714458
PMID: 29046400
Abstract
Transposons can impact the host genome by altering gene expression and participating in chromosome rearrangements. Therefore, organisms evolved different ways to minimize the level of transposition. In Saccharomyces cerevisiae and its close relative S. paradoxus, Ty1 copy number control (CNC) is mediated by the self-encoded restriction factor p22, which is derived from the GAG capsid gene and inhibits virus-like particle (VLP) assembly and function. Based on secondary screens of Ty1 cofactors, we identified LOC1, a RNA localization/ribosome biogenesis gene that affects Ty1 mobility predominantly in strains harboring Ty1 elements. Ribosomal protein mutants rps0b Delta and rpl7a Delta displayed similar CNC-specific phenotypes as loc1 Delta, suggesting that ribosome biogenesis is critical for CNC. The level of Ty1 mRNA and Ty1 internal (Ty1i) transcripts encoding p22 was altered in these mutants, and displayed a trend where the level of Ty1i RNA increased relative to full-length Ty1 mRNA. The level of p22 increased in these mutants, and the half-life of p22 also increased in a loc1D mutant. Transcriptomic analyses revealed small changes in the level of Ty1 transcripts or efficiency of translation initiation in a loc1D mutant. Importantly, a loc1D mutant had defects in assembly of Gag complexes and packaging Ty1 RNA. Our results indicate that defective ribosome biogenesis enhances CNC by increasing the level of p22, and raise the possibility for versatile links between VLP assembly, its cytoplasmic environment, and a novel stress response.
Details
- Title: Subtitle
- Ribosome Biogenesis Modulates Ty1 Copy Number Control in Saccharomyces cerevisiae
- Creators
- Hyo Won Ahn - University of GeorgiaJessica M. Tucker - University of GeorgiaJoshua A. Arribere - University of California, Santa CruzDavid J. Garfinkel - University of Georgia
- Resource Type
- Journal article
- Publication Details
- Genetics (Austin), Vol.207(4), pp.1441-1456
- DOI
- 10.1534/genetics.117.300388
- PMID
- 29046400
- PMCID
- PMC5714458
- NLM abbreviation
- Genetics
- ISSN
- 0016-6731
- eISSN
- 1943-2631
- Publisher
- Genetics Society America
- Number of pages
- 16
- Grant note
- R01GM095622 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) GM095622 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA University of Georgia Research Foundation Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Language
- English
- Date published
- 12/01/2017
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984297318102771
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