Rigor and reproducibility in human brain organoid research: Where we are and where we need to go

Soraya O Sandoval; Gerarda Cappuccio; Karina Kruth; Sivan Osenberg; Saleh M Khalil; Natasha M Méndez-Albelo; Krishnan Padmanabhan; Daifeng Wang; Mark J Niciu; Anita Bhattacharyya; Jason L Stein; André M M Sousa; Elisa A Waxman; Elizabeth D Buttermore; Dosh Whye; Carissa L Sirois; Aislinn Williams; Mirjana Maletic-Savatic; Xinyu Zhao; Cross-IDDRC Human Stem Cell Consortium

doi:10.1016/j.stemcr.2024.04.008

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Rigor and reproducibility in human brain organoid research: Where we are and where we need to go

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Rigor and reproducibility in human brain organoid research: Where we are and where we need to go

Soraya O Sandoval, Gerarda Cappuccio, Karina Kruth, Sivan Osenberg, Saleh M Khalil, Natasha M Méndez-Albelo, Krishnan Padmanabhan, Daifeng Wang, Mark J Niciu, Anita Bhattacharyya, …

Stem cell reports, Vol.19(6), pp.796-816

05/10/2024

DOI: 10.1016/j.stemcr.2024.04.008

PMCID: PMC11297560

PMID: 38759644

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Abstract

Human brain organoid models have emerged as a promising tool for studying human brain development and function. These models preserve human genetics and recapitulate some aspects of human brain development, while facilitating manipulation in an in vitro setting. Despite their potential to transform biology and medicine, concerns persist about their fidelity. To fully harness their potential, it is imperative to establish reliable analytic methods, ensuring rigor and reproducibility. Here, we review current analytical platforms used to characterize human forebrain cortical organoids, highlight challenges, and propose recommendations for future studies to achieve greater precision and uniformity across laboratories.

Details

Title: Subtitle: Rigor and reproducibility in human brain organoid research: Where we are and where we need to go
Creators: Soraya O Sandoval - University of Wisconsin–Madison
Gerarda Cappuccio - Texas Children's Hospital
Karina Kruth - University of Iowa Health Care
Sivan Osenberg - Texas Children's Hospital
Saleh M Khalil - Texas Children's Hospital
Natasha M Méndez-Albelo - University of Wisconsin–Madison
Krishnan Padmanabhan - University of Rochester
Daifeng Wang - University of Wisconsin–Madison
Mark J Niciu - University of Iowa
Anita Bhattacharyya - University of Wisconsin–Madison
Jason L Stein - University of North Carolina at Chapel Hill
André M M Sousa - University of Wisconsin–Madison
Elisa A Waxman - University of Pennsylvania
Elizabeth D Buttermore - Boston Children's Hospital
Dosh Whye - Boston Children's Hospital
Carissa L Sirois - University of Wisconsin–Madison
Aislinn Williams - University of Iowa Health Care
Mirjana Maletic-Savatic
Xinyu Zhao - University of Wisconsin–Madison
Cross-IDDRC Human Stem Cell Consortium
Resource Type: Journal article
Publication Details: Stem cell reports, Vol.19(6), pp.796-816
DOI: 10.1016/j.stemcr.2024.04.008
PMID: 38759644
PMCID: PMC11297560
NLM abbreviation: Stem Cell Reports
eISSN: 2213-6711
Language: English
Electronic publication date: 05/10/2024
Academic Unit: Psychiatry; Iowa Neuroscience Institute
Record Identifier: 9984628237502771

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