Journal article
Rigor and reproducibility in rodent behavioral research
Neurobiology of learning and memory, Vol.165, pp.106780-106780
11/2019
DOI: 10.1016/j.nlm.2018.01.001
PMID: 29307548
Abstract
•Strategies to maximize rigor and reproducibility in rodent behavioral assays.•Replicability of translational rodent models as preclinical research tools.•Key methods for one example, the novel object recognition cognitive test.•Extensive literature cited will refer the reader to detailed appropriate methods.
Behavioral neuroscience research incorporates the identical high level of meticulous methodologies and exacting attention to detail as all other scientific disciplines. To achieve maximal rigor and reproducibility of findings, well-trained investigators employ a variety of established best practices. Here we explicate some of the requirements for rigorous experimental design and accurate data analysis in conducting mouse and rat behavioral tests. Novel object recognition is used as an example of a cognitive assay which has been conducted successfully with a range of methods, all based on common principles of appropriate procedures, controls, and statistics. Directors of Rodent Core facilities within Intellectual and Developmental Disabilities Research Centers contribute key aspects of their own novel object recognition protocols, offering insights into essential similarities and less-critical differences. Literature cited in this review article will lead the interested reader to source papers that provide step-by-step protocols which illustrate optimized methods for many standard rodent behavioral assays. Adhering to best practices in behavioral neuroscience will enhance the value of animal models for the multiple goals of understanding biological mechanisms, evaluating consequences of genetic mutations, and discovering efficacious therapeutics.
Details
- Title: Subtitle
- Rigor and reproducibility in rodent behavioral research
- Creators
- Maria Gulinello - IDDRC Behavioral Core Facility, Neuroscience Department, Albert Einstein College of Medicine, Bronx, NY 10461, USAHeather A Mitchell - IDD Models Core, Waisman Center, University of Wisconsin Madison, Madison, WI 53705, USAQiang Chang - IDD Models Core, Waisman Center, University of Wisconsin Madison, Madison, WI 53705, USAW Timothy O'Brien - IDDRC Preclinical Models Core, Children’s Hospital of Philadelphia and University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USAZhaolan Zhou - IDDRC Preclinical Models Core, Children’s Hospital of Philadelphia and University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USATed Abel - IDDRC Preclinical Models Core, Children’s Hospital of Philadelphia and University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USALi Wang - IDDRC Neurobehavioral Core, Center for Neuroscience Research, Children's National Health System, Washington, DC 20010, USAJoshua G Corbin - IDDRC Neurobehavioral Core, Center for Neuroscience Research, Children's National Health System, Washington, DC 20010, USASurabi Veeraragavan - IDDRC Neurobehavioral Core, Baylor College of Medicine, Houston, TX 77030, USARodney C Samaco - IDDRC Neurobehavioral Core, Baylor College of Medicine, Houston, TX 77030, USANick A Andrews - IDDRC Neurodevelopmental Behavior Core, Boston Children’s Hospital, Boston, MA 02115, USAMichela Fagiolini - IDDRC Neurodevelopmental Behavior Core, Boston Children’s Hospital, Boston, MA 02115, USAToby B Cole - IDDRC Rodent Behavior Laboratory, Center on Human Development and Disability, University of Washington, Seattle, WA 98195, USAThomas M Burbacher - IDDRC Rodent Behavior Laboratory, Center on Human Development and Disability, University of Washington, Seattle, WA 98195, USAJacqueline N Crawley - IDDRC Rodent Behavior Core, MIND Institute, University of California Davis School of Medicine, Sacramento, CA 95817, USA
- Resource Type
- Journal article
- Publication Details
- Neurobiology of learning and memory, Vol.165, pp.106780-106780
- DOI
- 10.1016/j.nlm.2018.01.001
- PMID
- 29307548
- NLM abbreviation
- Neurobiol Learn Mem
- ISSN
- 1074-7427
- eISSN
- 1095-9564
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 11/2019
- Academic Unit
- Molecular Physiology and Biophysics; Psychiatry; Psychological and Brain Sciences; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Biochemistry and Molecular Biology
- Record Identifier
- 9984070529202771
Metrics
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