Role for Complement in Development of Helicobacter-Induced Gastritis in Interleukin-10-Deficient Mice

Hanan F Ismail; Juan Zhang; Richard G Lynch; Yi Wang; Daniel J Berg

doi:10.1128/IAI.71.12.7140-7148.2003

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Role for Complement in Development of Helicobacter-Induced Gastritis in Interleukin-10-Deficient Mice

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Role for Complement in Development of Helicobacter-Induced Gastritis in Interleukin-10-Deficient Mice

Hanan F Ismail, Juan Zhang, Richard G Lynch, Yi Wang and Daniel J Berg

Infection and immunity, Vol.71(12), pp.7140-7148

12/2003

DOI: 10.1128/IAI.71.12.7140-7148.2003

PMCID: PMC308887

PMID: 14638805

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Abstract

The mechanisms by which the immune response can eradicate gastric Helicobacter infection are unknown. We hypothesized that Helicobacter -induced activation of the complement system could promote both inflammation and eradication of Helicobacter from the stomach. In vitro studies demonstrated that Helicobacter felis activates complement in normal mouse serum but not in serum from Rag2 −/− mice, indicating that H. felis activates complement through the classical pathway. Next, we infected complement-depleted wild-type control and interleukin-10-deficient (IL-10 −/− ) mice with H. felis. Helicobacter infection of wild-type mice elicited a mild, focal gastritis and did not alter serum complement levels. Infection of IL-10 −/− mice with H. felis elicited severe gastritis. After the initial colonization, the IL-10 −/− mice completely cleared Helicobacter from the stomach by day 8. In contrast to wild-type mice, H. felis -infected IL-10 −/− mice had a marked increase in serum complement levels. Complement depletion of wild-type mice did not affect the intensity of gastric inflammation or the extent of Helicobacter colonization compared to that for the wild-type control mice. In contrast, complement depletion of Helicobacter -infected IL-10 −/− mice decreased the severity of gastritis, decreased the Helicobacter -induced infiltration of neutrophils into the stomach, and delayed the clearance of bacteria. In vitro studies of stimulated splenocytes and neutrophils from IL-10 −/− mice produced a twofold increase in complement production compared to that for wild-type mice. Pretreatment with IL-10 inhibited this increase. These studies identify a role for complement in the local immune response to gastric Helicobacter in IL-10 −/− mice and suggest a role for IL-10 in the regulation of complement production.

Host Response and Inflammation

Details

Title: Subtitle: Role for Complement in Development of Helicobacter-Induced Gastritis in Interleukin-10-Deficient Mice
Creators: Hanan F Ismail - Department of Internal Medicine
Juan Zhang - Department of Internal Medicine
Richard G Lynch - Department of Internal Medicine
Yi Wang - Department of Internal Medicine
Daniel J Berg - Department of Internal Medicine
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.71(12), pp.7140-7148
DOI: 10.1128/IAI.71.12.7140-7148.2003
PMID: 14638805
PMCID: PMC308887
NLM abbreviation: Infect Immun
ISSN: 0019-9567
eISSN: 1098-5522
Publisher: American Society for Microbiology
Language: English
Date published: 12/2003
Academic Unit: Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
Record Identifier: 9984094391202771

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