Journal article
Role for the Ran Binding Protein, Mog1p, in Saccharomyces cerevisiae SLN1-SKN7 Signal Transduction
Eukaryotic cell, Vol.3(6), pp.1544-1556
12/2004
DOI: 10.1128/EC.3.6.1544-1556.2004
PMCID: PMC539023
PMID: 15590828
Abstract
Yeast Sln1p is an osmotic stress sensor with histidine kinase activity. Modulation of Sln1 kinase activity in response to changes in the osmotic environment regulates the activity of the osmotic response mitogen-activated protein kinase pathway and the activity of the Skn7p transcription factor, both important for adaptation to changing osmotic stress conditions. Many aspects of Sln1 function, such as how kinase activity is regulated to allow a rapid response to the continually changing osmotic environment, are not understood. To gain insight into Sln1p function, we conducted a two-hybrid screen to identify interactors. Mog1p, a protein that interacts with the yeast Ran1 homolog, Gsp1p, was identified in this screen. The interaction with Mog1p was characterized in vitro, and its importance was assessed in vivo.
mog1
mutants exhibit defects in SLN1-SKN7 signal transduction and mislocalization of the Skn7p transcription factor. The requirement for Mog1p in normal localization of Skn7p to the nucleus does not fully account for the
mog1
-related defects in SLN1-SKN7 signal transduction, raising the possibility that Mog1p may play a role in Skn7 binding and activation of osmotic response genes.
Details
- Title: Subtitle
- Role for the Ran Binding Protein, Mog1p, in Saccharomyces cerevisiae SLN1-SKN7 Signal Transduction
- Creators
- Jade Mei-Yeh Lu - Department of Biological Sciences, Department of Biochemistry, University of Iowa, Iowa City, IowaRobert J Deschenes - Department of Biological Sciences, Department of Biochemistry, University of Iowa, Iowa City, IowaJan S Fassler - Department of Biological Sciences, Department of Biochemistry, University of Iowa, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Eukaryotic cell, Vol.3(6), pp.1544-1556
- DOI
- 10.1128/EC.3.6.1544-1556.2004
- PMID
- 15590828
- PMCID
- PMC539023
- NLM abbreviation
- Eukaryot Cell
- ISSN
- 1535-9778
- eISSN
- 1535-9786
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 12/2004
- Academic Unit
- Biology
- Record Identifier
- 9984217535902771
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