Role of DC-SIGN in the activation of dendritic cells by HPV-16 L1 virus-like particle vaccine

Alfonso J García-Piñeres; Allan Hildesheim; Matthew Trivett; Marcus Williams; Li Wu; Vineet N Kewalramani; Ligia A Pinto

doi:10.1002/eji.200535068

Back

Role of DC-SIGN in the activation of dendritic cells by HPV-16 L1 virus-like particle vaccine

Journal article

Open access

Peer reviewed

Role of DC-SIGN in the activation of dendritic cells by HPV-16 L1 virus-like particle vaccine

Alfonso J García-Piñeres, Allan Hildesheim, Matthew Trivett, Marcus Williams, Li Wu, Vineet N Kewalramani and Ligia A Pinto

European journal of immunology, Vol.36(2), pp.437-445

02/2006

DOI: 10.1002/eji.200535068

PMID: 16385626

Files and links (1)

url

https://doi.org/10.1002/eji.200535068View

Published (Version of record) Open Access

Abstract

Dendritic cell-specific intercellular adhesion molecule-grabbing non-integrin (DC-SIGN), a specific C-type lectin expressed on DC, binds and transmits different pathogens to susceptible cells. In the present study, we examined the role of DC-SIGN in the capture of human papillomavirus (HPV) pseudovirions and activation of DC. We demonstrate that HPV virus-like particles (VLP) bind to DC-SIGN expressed on transfected Raji cells and that antibodies against DC-SIGN block this interaction. DC take up VLP, which activate expression of costimulatory markers and cytokines/chemokines. Although our results indicate that DC-SIGN is not the major receptor for VLP in DC, this interaction contributes to the activation of DC surface antigens (HLA class I) and of various cytokines/chemokines, particularly TNF-alpha, IL-6, and RANTES. Induction of these markers in DC by VLP was significantly abrogated when binding to DC-SIGN was blocked by anti-DC-SIGN antibodies. These results suggest that DC-SIGN has a functional role in DC activation induced by HPV-16 L1-VLP, and thus highlight new aspects of DC interactions with HPV VLP.

Virion - immunology

Cell Adhesion Molecules - genetics

Antibodies, Monoclonal - pharmacology

Dendritic Cells - immunology

Humans

Lectins, C-Type - immunology

Cell Adhesion Molecules - immunology

Human papillomavirus 16 - immunology

Antigen Presentation - immunology

Lectins, C-Type - genetics

Receptors, Cell Surface - immunology

Transfection

Antigen Presentation - drug effects

Viral Vaccines - immunology

Antibodies, Monoclonal - immunology

Cytokines - immunology

Papillomavirus Vaccines

Receptors, Cell Surface - genetics

Details

Title: Subtitle: Role of DC-SIGN in the activation of dendritic cells by HPV-16 L1 virus-like particle vaccine
Creators: Alfonso J García-Piñeres - SAIC-Frederick/NCI-Frederick, Frederick, MD, USA
Allan Hildesheim
Matthew Trivett
Marcus Williams
Li Wu
Vineet N Kewalramani
Ligia A Pinto
Resource Type: Journal article
Publication Details: European journal of immunology, Vol.36(2), pp.437-445
Publisher: Germany
DOI: 10.1002/eji.200535068
PMID: 16385626
ISSN: 0014-2980
eISSN: 1521-4141
Grant note: N01-CO-12400 / NCI NIH HHS
Language: English
Date published: 02/2006
Academic Unit: Microbiology and Immunology
Record Identifier: 9984002380002771

Metrics

26 Record Views

20 Times Cited - Web of Science