Role of Drosophila HP1 in euchromatic gene expression

Diane E Cryderman; Stephanie K Grade; Yuhong Li; Laura Fanti; Sergio Pimpinelli; Lori L Wallrath

doi:10.1002/dvdy.20310

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Role of Drosophila HP1 in euchromatic gene expression

Journal article

Peer reviewed

Role of Drosophila HP1 in euchromatic gene expression

Diane E Cryderman, Stephanie K Grade, Yuhong Li, Laura Fanti, Sergio Pimpinelli and Lori L Wallrath

Developmental dynamics, Vol.232(3), pp.767-774

03/2005

DOI: 10.1002/dvdy.20310

PMID: 15704177

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Abstract

Heterochromatin protein 1 (HP1), a gene silencing protein, localizes to centric heterochromatin through an interaction with methylated K9 of histone H3, a modification generated by the histone methyl transferase SU(VAR)3-9. On Drosophila polytene chromosomes, HP1 also localizes to 200 sites scattered throughout euchromatin. To address the role of HP1 in euchromatic gene regulation, mRNAs from wild-type and Su(var)2-5 mutants lacking HP1 were compared. Genes residing within a 550-kb genomic region enriched in HP1 that show altered expression in the Su(var)2-5 mutant were analyzed in detail. Three genes within this region, Pros35, CG5676, and cdc2, were found to associate with HP1 by chromatin immunoprecipitation. Surprisingly, these genes require HP1 for expression, suggesting a positive role for HP1 in euchromatic gene expression. Of these genes, only cdc2 is packaged with methylated K9 H3. Furthermore, none of the genes show altered expression in a Su(var)3-9 mutant. Collectively, these data demonstrate multiple mechanisms for HP1 localization within euchromatin and show that some genes associated with HP1 are not affected by alterations in Su(var)3-9 dosage.

Gene Expression

Animals

Chromosomal Proteins, Non-Histone - metabolism

Heterochromatin - metabolism

Gene Silencing

Gene Dosage

Drosophila - metabolism

Drosophila Proteins - metabolism

Drosophila - genetics

Euchromatin - metabolism

Details

Title: Subtitle: Role of Drosophila HP1 in euchromatic gene expression
Creators: Diane E Cryderman - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
Stephanie K Grade
Yuhong Li
Laura Fanti
Sergio Pimpinelli
Lori L Wallrath
Resource Type: Journal article
Publication Details: Developmental dynamics, Vol.232(3), pp.767-774
DOI: 10.1002/dvdy.20310
PMID: 15704177
NLM abbreviation: Dev Dyn
ISSN: 1058-8388
eISSN: 1097-0177
Publisher: United States
Grant note: GM16153 / NIGMS NIH HHS
Language: English
Date published: 03/2005
Academic Unit: Biochemistry and Molecular Biology; University College Courses
Record Identifier: 9984025281302771

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