Journal article
Role of FcRγ and factor XIIIA in coated platelet formation
Blood, Vol.106(13), pp.4146-4151
12/15/2005
DOI: 10.1182/blood-2005-03-1223
PMCID: PMC1895237
PMID: 16105983
Abstract
Platelet activation in response to dual stimulation with collagen and thrombin results in the formation of a subpopulation of activated platelets known as coated platelets. Coated platelets are characterized by high surface levels of α-granule proteins and phosphatidylserine, which support the assembly of procoagulant protein complexes. Using murine models, we tested the hypothesis that the collagen receptor-associated molecule FcRγ and the transglutaminase factor XIIIA are required for the formation of coated platelets. Following dual stimulation with the collagen receptor agonist convulxin and thrombin, 68% of platelets from C57BL/6 mice acquired the coated platelet phenotype, defined by high surface levels of fibrinogen and von Willebrand factor and decreased binding of the αIIbβ3 activation-dependent antibody PE-JON/A. In FcRγ
-/-
mice, only 10% of platelets became “coated” after dual stimulation with convulxin plus thrombin (
P
< .05 vs C57BL/6 platelets). Decreased coated platelet formation in FcRγ
-/-
platelets was accompanied by decreased annexin V binding (
P
< .01) and decreased platelet procoagulant activity (
P
< .05). Platelets from FXIIIA
-/-
mice did not differ from control platelets in coated platelet formation or annexin V binding. We conclude that FcRγ, but not factor XIIIA, is essential for formation of highly procoagulant coated platelets following dual stimulation with collagen and thrombin.
Details
- Title: Subtitle
- Role of FcRγ and factor XIIIA in coated platelet formation
- Creators
- Shawn M Jobe - From the Departments of Pediatrics, Internal Medicine, and Urology, University of Iowa College of Medicine, Iowa City, IA; ZLB Behring, Marburg, Germany; and Veterans Affairs Medical Center, Iowa City, IALorie Leo - From the Departments of Pediatrics, Internal Medicine, and Urology, University of Iowa College of Medicine, Iowa City, IA; ZLB Behring, Marburg, Germany; and Veterans Affairs Medical Center, Iowa City, IAJoshua S Eastvold - From the Departments of Pediatrics, Internal Medicine, and Urology, University of Iowa College of Medicine, Iowa City, IA; ZLB Behring, Marburg, Germany; and Veterans Affairs Medical Center, Iowa City, IAGerhard Dickneite - From the Departments of Pediatrics, Internal Medicine, and Urology, University of Iowa College of Medicine, Iowa City, IA; ZLB Behring, Marburg, Germany; and Veterans Affairs Medical Center, Iowa City, IATimothy L Ratliff - From the Departments of Pediatrics, Internal Medicine, and Urology, University of Iowa College of Medicine, Iowa City, IA; ZLB Behring, Marburg, Germany; and Veterans Affairs Medical Center, Iowa City, IASteven R Lentz - From the Departments of Pediatrics, Internal Medicine, and Urology, University of Iowa College of Medicine, Iowa City, IA; ZLB Behring, Marburg, Germany; and Veterans Affairs Medical Center, Iowa City, IAJorge Di Paola - From the Departments of Pediatrics, Internal Medicine, and Urology, University of Iowa College of Medicine, Iowa City, IA; ZLB Behring, Marburg, Germany; and Veterans Affairs Medical Center, Iowa City, IA
- Resource Type
- Journal article
- Publication Details
- Blood, Vol.106(13), pp.4146-4151
- Publisher
- 2005 by The American Society of Hematology
- DOI
- 10.1182/blood-2005-03-1223
- PMID
- 16105983
- PMCID
- PMC1895237
- ISSN
- 0006-4971
- eISSN
- 1528-0020
- Language
- English
- Date published
- 12/15/2005
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Urology; Internal Medicine
- Record Identifier
- 9984094371402771
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