Journal article
Role of Leukocytes in Coronary Vascular Endothelial Injury Due to Ischemia and Reperfusion
Circulation research, Vol.69(6), pp.1566-1574
12/1991
DOI: 10.1161/01.RES.69.6.1566
PMID: 1954676
Abstract
A possible cause of the coronary endothelial injury that occurs with ischemia and reperfusion is the local accumulation of leukocytes during these events. To investigate the role of leukocytes in coronary endothelial injury, we tested the effect of leukocyte removal by filtering on coronary endothelial function in a canine model of regional myocardial ischemia and reperfusion. Blood was supplied to the left anterior descending and circumflex arteries of anesthetized dogs via an extracorporeal circulation. A 60-minute left anterior descending occlusion was followed by 120 minutes of reperfusion either with (n=6) or without (n=6) leukocyte filters in the extracorporeal circuit. Regional myocardial blood flow was measured with radiolabeled microspheres. Radiolabeled autologous transferrin (In) and erythrocytes (Tc) were given intravenously during reperfusion for assessment of microvascular permeability. Left anterior descending and circumflex coronary artery rings were assessed in vitro for endothelium-dependent dilation to acetylcholine, ADP, and thrombin. In unfiltered dogs, ischemia and reperfusion increased the protein leak index of ischemic myocardium 2.3-fold compared with that of nonischemic myocardium (2.3±0.5 to 5.2±1.6, p <0.05). In filtered dogs, there was no difference in the protein leak index of nonischemic versus ischemic myocardium (1.5±0.4 versus 1.9±0.5, p=NS). There was impaired left anterior descending coronary artery relaxation (versus circumflex) in response to endothelium-dependent vasodilators in vitro. However, relaxation was not consistently improved by leukocyte filtering. We conclude that leukocytes are responsible for the endothelial injury secondary to ischemia and reperfusion in the coronary microvasculature but have little or no effect on the endothelial injury in epicardial coronary arteries.
Details
- Title: Subtitle
- Role of Leukocytes in Coronary Vascular Endothelial Injury Due to Ischemia and Reperfusion
- Creators
- Frank Sheridan - University of Colorado HealthIra Dauber - University of Colorado HealthIvan McMurtry - University of Colorado HealthEdward Lesnefsky - University of Colorado HealthLawrence Horwitz - University of Colorado Health
- Resource Type
- Journal article
- Publication Details
- Circulation research, Vol.69(6), pp.1566-1574
- Publisher
- American Heart Association, Inc
- DOI
- 10.1161/01.RES.69.6.1566
- PMID
- 1954676
- ISSN
- 0009-7330
- eISSN
- 1524-4571
- Language
- English
- Date published
- 12/1991
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984656527702771
Metrics
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