Journal article
Role of N-Linked Glycosylation in PKR2 Trafficking and Signaling
Frontiers in neuroscience, Vol.15, 730417
08/01/2021
DOI: 10.3389/fnins.2021.730417
PMCID: PMC8414166
PMID: 34483834
Abstract
Prokineticin receptors are GPCRs involved in several physiological processes including the regulation of energy homeostasis, nociception, and reproductive function. PKRs are inhibited by the endogenous accessory protein MRAP2 which prevents them from trafficking to the plasma membrane. Very little is known about the importance of post-translational modification of PKRs and their role in receptor trafficking and signaling. Here we identify 2 N-linked glycosylation sites within the N-terminal region of PKR2 and demonstrate that glycosylation of PKR2 at position 27 is important for its plasma membrane localization and signaling. Additionally, we show that glycosylation at position 7 results in a decrease in PKR2 signaling through Gαs without impairing Gαq/11 signaling.
Details
- Title: Subtitle
- Role of N-Linked Glycosylation in PKR2 Trafficking and Signaling
- Creators
- Jissele A. Verdinez - Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, IA, United StatesJulien A. Sebag - Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, IA, United States
- Resource Type
- Journal article
- Publication Details
- Frontiers in neuroscience, Vol.15, 730417
- DOI
- 10.3389/fnins.2021.730417
- PMID
- 34483834
- PMCID
- PMC8414166
- NLM abbreviation
- Front Neurosci
- ISSN
- 1662-453X
- eISSN
- 1662-453X
- Publisher
- Frontiers Media S.A
- Grant note
- DOI: 10.13039/100005440, name: Center for Scientific Review
- Language
- English
- Date published
- 08/01/2021
- Academic Unit
- Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984120580202771
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