Journal article
Role of Proteolytic Enzyme Inhibitors on Carious and Eroded Dentin Associated With a Universal Bonding System
Operative dentistry, Vol.42(6), pp.E188-E196
11/01/2017
DOI: 10.2341/16-178-L
PMID: 29144877
Abstract
SUMMARY Objectives: The aim of this study was to evaluate the effect of proteolytic inhibitors on the bond strength of a universal adhesive system (etch-and-rinse mode) applied to artificial carious and eroded dentin. Methods: Ninety molars were prepared and randomly divided into three groups according to the substrate: N, no challenges; ACD, artificial carious dentin simulation and ERO, artificial erosion simulation with orange juice. All groups were redivided into three subgroups according to the dentin pretreatment: W, water; CHX, 2% digluconate chlorhexidine; and E-64 (trans-epoxysuccinyl-L-leucylamido-[4-guanidino] butane), 5 μM E-64 inhibitor. They constituted a total of nine groups (n=10): N-W, N-CHX, N-E64, ACD-W, ACD-CHX, ACD-E64, ERO-W, ERO-CHX, and ERO-E64. All specimens were restored with Adper Single Bond Universal/Filtek Z250. Beams (0.64 mm2) were obtained and subjected to the microtensile test (μTBS) in a universal testing machine at 0.5 mm/min. The failure mode of the interfaces was determined by optical microscopy (40× magnification). Data were statistically analyzed by three-way analysis of variance and Tukey tests (p<0.05). Results: All individual factors (p<0.0001) and the interaction between substrate and treatment (p=0.0011) and between substrate and time (p=0.0003) were statistically significant. The caries substrate contributed negatively to bond strength. Chlorhexidine reduced bond strength for normal and eroded conditions. Only the normal substrate was negatively affected by time despite the pretreatment. Conclusions: The universal bonding system appears to be a promising bonding strategy for the maintenance of bond strength to affected dentin. E-64 did not affect bonding to the dentin in contrast to the use of chlorhexidine, which, when associated with the universal system, did affect the microtensile bond strength for artificial carious dentin.
Details
- Title: Subtitle
- Role of Proteolytic Enzyme Inhibitors on Carious and Eroded Dentin Associated With a Universal Bonding System
- Creators
- M C Giacomini - Marina Ciccone Giacomini, DDS, MS, PhD student, Department of Operative Dentistry, Endodontics, and Dental Materials, Bauru School of Dentistry, University of São Paulo, São Paulo, BrazilPMC Scaffa - Polliana Mendes Candia Scaffa, DDS, MS, PhD, postdoctoral student, Department of Biological Science, Bauru School of Dentistry, University of São Paulo, São Paulo, BrazilL P Chaves - Larissa Pinceli Chaves, DDS, MS, PhD, Department of Operative Dentistry, Endodontics, and Dental Materials, Bauru School of Dentistry, University of São Paulo, São Paulo, BrazilCMP Vidal - Cristina de Mattos Pimenta Vidal, DDS, MS, PhD, assistant professor, Department of Operative Dentistry, College of Dentistry, University of Iowa, Iowa City, IA, USAT N Machado - Thaís Nayara Machado, DDS, Department of Operative Dentistry, Endodontics, and Dental Materials, Bauru School of Dentistry, University of São Paulo, São Paulo, BrazilH M Honório - Heitor Marques Honório, DDS, MS, PhD, associate professor, Department of Pediatric Dentistry, Orthodontics, and Collective Health, Bauru School of Dentistry, University of São Paulo, São Paulo, BrazilL TjäderhaneL Wang - Linda Wang, DDS, MS, PhD, associate professor, Department of Operative Dentistry, Endodontics, and Dental Materials, Bauru School of Dentistry, University of São Paulo, São Paulo, Brazil
- Resource Type
- Journal article
- Publication Details
- Operative dentistry, Vol.42(6), pp.E188-E196
- DOI
- 10.2341/16-178-L
- PMID
- 29144877
- ISSN
- 0361-7734
- eISSN
- 1559-2863
- Language
- English
- Date published
- 11/01/2017
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984065709602771
Metrics
15 Record Views