Logo image
Back
Role of amines in anaphylactic contraction of guinea pig isolated smooth muscle
Journal article   Peer reviewed

Role of amines in anaphylactic contraction of guinea pig isolated smooth muscle

P. D Joiner, M Wall, L. B Davis and F Hahn
The Journal of Allergy and Clinical Immunology, Vol.53(5), pp.261-270
1974
DOI: 10.1016/0091-6749(74)90104-3
PMID: 4150792

View Online

Abstract

The possible role of acetylcholine (ACh,) histamine (His), and 5-hydroxytryptamine (5-HT) in the contractile response to ovalbumin (Oval) of ileal and tracheal muscle from sensitized guinea pigs was examined. Antagonists to these agents (atropine, mepyramine and methysergide, 5-benzyloxygramine, or 5-HT tachyphylaxis, respectively) were employed singly or in combinations. Care was always taken to ensure specificity of blockade. Results indicate that about half of the peak magnitude of the oval-induced contraction is absent in the presence of a specific inhibitory concentration of mepyramine. This finding suggests that His plays a considerable role in the oval-induced contraction. Contrary to previous reports, similar evidence was not obtained of a role for ACh and 5-HT in the Oval-induced contraction. The source of the agonists released by Oval was also investigated. Pretreatment with compound48/80 almost completely eliminated the Oval-induced contraction of ileal muscle while reducing the tracheal contraction about one-half. It would appear that the Oval-induced ileal contraction involves primarily agonists released from mast cells in the preparation whereas tracheal contraction is only partly dependent on mediators from mast cells.

Details

Metrics

21 Record Views
30 Times Cited - Web of Science
Logo image