Role of c-ABL in DENV-2 Infection and Actin Remodeling in Vero Cells

Grace Paola Carreño-Flórez; Alexandra Milena Cuartas-López; Ryan L. Boudreau; Miguel Vicente-Manzanares; Juan Carlos Gallego-Gómez

doi:10.3390/ijms26094206

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Role of c-ABL in DENV-2 Infection and Actin Remodeling in Vero Cells

Journal article

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Role of c-ABL in DENV-2 Infection and Actin Remodeling in Vero Cells

Grace Paola Carreño-Flórez, Alexandra Milena Cuartas-López, Ryan L. Boudreau, Miguel Vicente-Manzanares and Juan Carlos Gallego-Gómez

International journal of molecular sciences, Vol.26(9), 4206

04/29/2025

DOI: 10.3390/ijms26094206

PMCID: PMC12071696

PMID: 40362443

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Abstract

In this study, we address the role of c-ABL (cellular Abelson Tyr kinase) in the cytoskeletal rearrangements induced by DENV (Dengue virus) infection in mammalian cells. Using the specific inhibitor imatinib and targeted RNA interference, we show that c-ABL is necessary for viral entry and subsequent ENV (DENV envelope protein) accumulation in infected cells. In addition, c-ABL targeting attenuates F-actin reorganization induced by DENV infection. Together with the involvement of c-ABL in endothelial dysfunction induced by DENV and host secreted factors, our findings strongly suggest that c-ABL is a potential host-targeted antiviral that could control DENV infection and/or its evolution to more severe forms of the disease.

DENV

c-ABL

cellular Abelson Tyr kinase

actin

imatinib

artificial microRNA

Details

Title: Subtitle: Role of c-ABL in DENV-2 Infection and Actin Remodeling in Vero Cells
Creators: Grace Paola Carreño-Flórez
Alexandra Milena Cuartas-López
Ryan L. Boudreau
Miguel Vicente-Manzanares
Juan Carlos Gallego-Gómez
Resource Type: Journal article
Publication Details: International journal of molecular sciences, Vol.26(9), 4206
DOI: 10.3390/ijms26094206
PMID: 40362443
PMCID: PMC12071696
NLM abbreviation: Int J Mol Sci
ISSN: 1422-0067
eISSN: 1422-0067
Publisher: MDPI
Grant note: Colombian Ministerio de Ciencia, Tecnologia e Innovacion (Minciencias): 111584466951 CODI-UdeA: 2020-34137 Spanish Ministry of Science and Innovation: PID2020-116232RB-I00, PID2023-153018NB-I00 NIH NHLBI: R01 HL148796
This research was funded by the Colombian Ministerio de Ciencia, Tecnologia e Innovacion (Minciencias) grant 111584466951, and CODI-UdeA 2020-34137. M.V.-M. is funded by the Spanish Ministry of Science and Innovation [grants PID2020-116232RB-I00 and PID2023-153018NB-I00]. RLB is funded by NIH NHLBI R01 HL148796.
Language: English
Date published: 04/29/2025
Academic Unit: Iowa Neuroscience Institute; Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
Record Identifier: 9984820567402771

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