Journal article
Role of nitric oxide and adenosine in the onset of vasodilation during dynamic forearm exercise
European journal of applied physiology, Vol.113(2), pp.295-303
02/2013
DOI: 10.1007/s00421-012-2439-0
PMID: 22692759
Abstract
We tested the hypothesis that nitric oxide (NO) and adenosine contribute to the onset of vasodilation during dynamic forearm exercise. Twenty-two subjects performed rhythmic forearm exercise (20 % of maximum) during control and NO synthase (NOS) inhibition (N (G)-monomethyl-L-arginine; L-NMMA) trials. A subset of subjects performed a third trial of forearm exercise during combined inhibition of NOS and adenosine (aminophylline; n = 9). Additionally, a separate group of subjects (n = 7) performed rhythmic forearm exercise during control, inhibition of adenosine alone and combined inhibition of adenosine and NOS. Forearm vascular conductance (FVC; ml min(-1) · 100 mmHg(-1)) was calculated from blood flow and mean arterial pressure (mmHg). The onset of vasodilation was assessed by calculating the slope of the FVC response for every duty cycle between baseline and steady state, and the number of duty cycles (1-s contraction/2-s relaxation) to reach steady state. NOS inhibition blunted vasodilation at the onset of exercise (11.1 ± 0.8 vs. 8.5 ± 0.6 FVC units/duty cycle; P < 0.001 vs. control) and increased the time to reach steady state (25 ± 1 vs. 32 ± 1 duty cycles; P < 0.001 vs. control). Vasodilation was blunted further with combined inhibition of NOS and adenosine (7.5 ± 0.6 vs. 6.2 ± 0.8 FVC units/duty cycle; P < 0.05 vs. L-NMMA alone), but not with aminophylline alone (16.0 ± 2.2 vs. 14.7 ± 2.0 FVC units/duty cycle; P = 0.67 vs. control). Our data indicate that NO and adenosine (in the absence of NO) contribute to the onset of vasodilation during dynamic forearm exercise.
Details
- Title: Subtitle
- Role of nitric oxide and adenosine in the onset of vasodilation during dynamic forearm exercise
- Creators
- Darren P Casey - Department of Anesthesiology, Mayo Clinic, 200 First St. SW, Rochester, MN 55905, USA. casey.darren@mayo.eduEssa A MohamedMichael J Joyner
- Resource Type
- Journal article
- Publication Details
- European journal of applied physiology, Vol.113(2), pp.295-303
- DOI
- 10.1007/s00421-012-2439-0
- PMID
- 22692759
- ISSN
- 1439-6319
- eISSN
- 1439-6327
- Grant note
- AR-55819 / NIAMS NIH HHS RR-024150 / NCRR NIH HHS HL-46493 / NHLBI NIH HHS
- Language
- English
- Date published
- 02/2013
- Academic Unit
- Physical Therapy and Rehabilitation Science; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984083885502771
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