Role of phytocystatin in combating metal ion induced conformational alterations in glutathione reductase

Azaj Ahmed; Anas Shamsi; Moyad Jamal Shahwan; Fakhra Amin; Bilqees Bano

doi:10.1016/j.ijbiomac.2019.01.055

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Role of phytocystatin in combating metal ion induced conformational alterations in glutathione reductase

Journal article

Peer reviewed

Role of phytocystatin in combating metal ion induced conformational alterations in glutathione reductase

Azaj Ahmed, Anas Shamsi, Moyad Jamal Shahwan, Fakhra Amin and Bilqees Bano

International journal of biological macromolecules, Vol.127, pp.271-277

04/15/2019

DOI: 10.1016/j.ijbiomac.2019.01.055

PMID: 30654031

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Abstract

Glutathione reductase (GR) is a flavoprotein that catalyses the reduction of oxidized glutathione (GSSG) to reduced glutathione (2GSH) in the presence of coenzyme NADPH. The importance of glutathione stems from the fact that it serves an important role in various metabolic processes. Plants growing in highly polluted areas are exposed to higher concentration of metal ions; thereby feeling abiotic stress and affecting various regulatory enzyme activities. In this study, effect of metal ions has been studied on GR Phytocystatins show an increased expression in abiotic stress conditions. Herein, the effect of cystatin isolated from yellow mustard seeds (YMP) on heavy metals induced conformational changes in GR was investigated making use of GR activity assay, UV-absorption spectroscopy, fluorescence spectroscopy, FTIR, CD, ITC and SEM analysis. The results obtained clearly reveals that metal ions like Cu2+ and Zn+2 induces concentration dependent conformational changes in GR; YMP restores these alterations in way decreasing the effective concentration of metal ions. (C) 2019 Elsevier B.V. All rights reserved.

Biochemistry & Molecular Biology

Chemistry

Chemistry, Applied

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Details

Title: Subtitle: Role of phytocystatin in combating metal ion induced conformational alterations in glutathione reductase
Creators: Azaj Ahmed - Aligarh Muslim University
Anas Shamsi - Aligarh Muslim University
Moyad Jamal Shahwan - Ajman University
Fakhra Amin - Aligarh Muslim University
Bilqees Bano - Aligarh Muslim University
Resource Type: Journal article
Publication Details: International journal of biological macromolecules, Vol.127, pp.271-277
DOI: 10.1016/j.ijbiomac.2019.01.055
PMID: 30654031
NLM abbreviation: Int J Biol Macromol
ISSN: 0141-8130
eISSN: 1879-0003
Publisher: Elsevier
Number of pages: 7
Language: English
Date published: 04/15/2019
Academic Unit: Internal Medicine
Record Identifier: 9985177946602771

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