Journal article
Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence
The Journal of infectious diseases, Vol.210(12), pp.1951-1961
12/15/2014
DOI: 10.1093/infdis/jiu299
PMCID: PMC6281352
PMID: 24850789
Abstract
Lipid bodies (LB; lipid droplets) are cytoplasmic organelles involved in lipid metabolism. Mammalian LBs display an important role in host-pathogen interactions, but the role of parasite LBs in biosynthesis of prostaglandin F2α (PGF2α) has not been investigated. We report herein that LBs increased in abundance during development of Leishmania infantum chagasi to a virulent metacyclic stage, as did the expression of PGF2α synthase (PGFS). The amount of parasite LBs and PGF2α were modulated by exogenous arachidonic acid. During macrophage infection, LBs were restricted to parasites inside the parasitophorous vacuoles (PV). We detected PGF2α receptor (FP) on the Leishmania PV surface. The blockage of FP with AL8810, a selective antagonist, hampered Leishmania infection, whereas the irreversible inhibition of cyclooxygenase with aspirin increased the parasite burden. These data demonstrate novel functions for parasite-derived LBs and PGF2α in the cellular metabolism of Leishmania and its evasion of the host immune response.
Details
- Title: Subtitle
- Role of prostaglandin F2α production in lipid bodies from Leishmania infantum chagasi: insights on virulence
- Creators
- Théo Araújo-Santos - Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (FIOCRUZ) Federal University of Bahia (UFBA), Salvador, Bahia, Brazil University of Iowa and the Iowa City VA Medical Center, IowaNilda E Rodríguez - University of Iowa and the Iowa City VA Medical Center, IowaSara Moura-Pontes - Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (FIOCRUZ) Federal University of Bahia (UFBA), Salvador, Bahia, BrazilUpasna Gaur Dixt - University of Iowa and the Iowa City VA Medical Center, IowaDaniel R Abánades - Department of Chemical and Physical Biology, Centro de Investigaciones Biológicas, CSIC, Madrid, SpainPatrícia T Bozza - Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de JaneiroMary E Wilson - University of Iowa and the Iowa City VA Medical Center, IowaValéria Matos Borges - Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (FIOCRUZ) Federal University of Bahia (UFBA), Salvador, Bahia, Brazil Institute for Investigation in Immunology, iii-INCT (National Institute of Science and Technology), São Paulo, Brazil
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.210(12), pp.1951-1961
- Publisher
- United States
- DOI
- 10.1093/infdis/jiu299
- PMID
- 24850789
- PMCID
- PMC6281352
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Grant note
- R01 AI045540 / NIAID NIH HHS I01 BX001983 / BLRD VA
- Language
- English
- Date published
- 12/15/2014
- Academic Unit
- Microbiology and Immunology; International Programs; Epidemiology; Internal Medicine
- Record Identifier
- 9984001152502771
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