Journal article
Role of respiratory NADH oxidation in the regulation of Staphylococcus aureus virulence
EMBO reports, Vol.21(5), 45832
05/06/2020
DOI: 10.15252/embr.201845832
PMCID: PMC7202225
PMID: 32202364
Abstract
The success of Staphylococcus aureus as a pathogen is due to its capability of fine-tuning its cellular physiology to meet the challenges presented by diverse environments, which allows it to colonize multiple niches within a single vertebrate host. Elucidating the roles of energy-yielding metabolic pathways could uncover attractive therapeutic strategies and targets. In this work, we seek to determine the effects of disabling NADH-dependent aerobic respiration on the physiology of S. aureus. Differing from many pathogens, S. aureus has two type-2 respiratory NADH dehydrogenases (NDH-2s) but lacks the respiratory ion-pumping NDHs. Here, we show that the NDH-2s, individually or together, are not essential either for respiration or growth. Nevertheless, their absence eliminates biofilm formation, production of alpha-toxin, and reduces the ability to colonize specific organs in a mouse model of systemic infection. Moreover, we demonstrate that the reason behind these phenotypes is the alteration of the fatty acid metabolism. Importantly, the SaeRS two-component system, which responds to fatty acids regulation, is responsible for the link between NADH-dependent respiration and virulence in S. aureus.
Details
- Title: Subtitle
- Role of respiratory NADH oxidation in the regulation of Staphylococcus aureus virulence
- Creators
- Lici A. Schurig-Briccio - University of Illinois Urbana-ChampaignPaola K. Parraga Solorzano - University of Illinois Urbana-ChampaignAndrea M. Lencina - University of Illinois Urbana-ChampaignJana N. Radin - University of Illinois Urbana-ChampaignGrischa Y. Chen - University of Wisconsin–MadisonJohn-Demian Sauer - University of Wisconsin–MadisonThomas E. Kehl-Fie - University of Illinois Urbana-ChampaignRobert B. Gennis - University of Illinois Urbana-Champaign
- Resource Type
- Journal article
- Publication Details
- EMBO reports, Vol.21(5), 45832
- Publisher
- Wiley
- DOI
- 10.15252/embr.201845832
- PMID
- 32202364
- PMCID
- PMC7202225
- ISSN
- 1469-221X
- eISSN
- 1469-3178
- Number of pages
- 15
- Grant note
- Basil O'Connor Starter Scholar Award from March of Dimes R01 GM095600; R01 HL16101; K22 AI104805; R01 AI118880; R01 CA188034 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 05/06/2020
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984618643302771
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