Journal article
Role of salivary epithelial toll-like receptors 2 and 4 in modulating innate immune responses in chronic periodontitis
Journal of periodontal research, Vol.48(6), pp.757-765
12/01/2013
DOI: 10.1111/jre.12066
PMID: 23679005
Abstract
Background and ObjectiveChronic periodontitis is initiated by sequential colonization with a broad array of bacteria and is perpetuated by an immune-inflammatory response to the changing biofilm. Host recognition of microbes is largely mediated by toll-like receptors (TLRs), which interact with conserved pathogen-associated molecular patterns. Based on ligand recognition, TLR-2 and TLR-4 interact with most periodontal pathogens. Extracrevicular bacterial reservoirs, such as the oral epithelial cells, contribute to the persistence of periodontitis. Human saliva is a rich source of oral epithelial cells that express functional TLRs. In this study we investigated the role of salivary epithelial cell (SEC) TLR-2 and TLR-4 in patients with generalized chronic periodontitis.
Material and MethodsUnstimulated whole saliva (UWS) was collected from patients with generalized chronic periodontitis and from healthy individuals after obtaining informed consent. Epithelial cells isolated from each UWS sample were assessed for TLR-2, TLR-4, peptidoglycan recognition protein (PGRP)-3 and PGRP-4 by quantitative real-time PCR. In addition, the SECs were stimulated in vitro with microbial products for up to 24h. The culture supernatant was assessed for cytokines by ELISA.
ResultsStimulation with TLR-2- or TLR-4-specific ligands induced cytokine secretion with differential kinetics and up-regulated TLR2 and TLR4 mRNAs, respectively, in cultures of SECs from patients with periodontitis. In addition, the SECs from patients with periodontitis exhibited reduced PGRP3 and PGRP4 mRNAs, the TLR-responsive genes with antibacterial properties.
ConclusionSECs derived from the UWS of patients with chronic periodontitis are phenotypically distinct and could represent potential resources for assessing the epithelial responses to periodontal pathogens in the course of disease progression and persistence.
Details
- Title: Subtitle
- Role of salivary epithelial toll-like receptors 2 and 4 in modulating innate immune responses in chronic periodontitis
- Creators
- V. Swaminathan - Indiana UniversityS. Prakasam - Indiana UniversityV. Puri - Indiana UniversityM. Srinivasan - Indiana University – Purdue University Indianapolis
- Resource Type
- Journal article
- Publication Details
- Journal of periodontal research, Vol.48(6), pp.757-765
- Publisher
- Wiley
- DOI
- 10.1111/jre.12066
- PMID
- 23679005
- ISSN
- 0022-3484
- eISSN
- 1600-0765
- Number of pages
- 9
- Grant note
- Indiana University of School of Dentistry student research subcommittee
- Language
- English
- Date published
- 12/01/2013
- Academic Unit
- Periodontics
- Record Identifier
- 9984446515802771
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