Role of the major histocompatibility complex class II transmembrane region in antigen presentation and intracellular trafficking

Yelena A Barabanova; Hee-Kap Kang; Jinjong Myoung; Bongsu Kang; Gail A Bishop; Byung S Kim

doi:10.1111/j.0019-2805.2003.01772.x

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Role of the major histocompatibility complex class II transmembrane region in antigen presentation and intracellular trafficking

Journal article

Open access

Peer reviewed

Role of the major histocompatibility complex class II transmembrane region in antigen presentation and intracellular trafficking

Yelena A Barabanova, Hee-Kap Kang, Jinjong Myoung, Bongsu Kang, Gail A Bishop and Byung S Kim

Immunology, Vol.111(2), pp.165-172

02/2004

DOI: 10.1111/j.0019-2805.2003.01772.x

PMCID: PMC1782412

PMID: 15027901

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Abstract

While a sorting signal in the cytoplasmic tail of the major histocompatibility complex (MHC) class II molecules is known to influence their endocytic transport, potential effects of the transmembrane (TM) domain of the MHC class II molecules on endocytic transport remain unclear. We have examined the role of the TM domain by comparing antigen-presenting functions of the wildtype (WT) I-Ab and mutant (MT) I-Ab molecule substituted in the beta-chain TM with alpha chain TM. A20 cells transfected with WT I-Ab were able to present antigen (hen egg lysozyme) better to some hybridomas, while those transfected with MT I-Ab consistently outperformed WT for other hybridomas recognizing different epitopes. This difference in antigen processing and presentation is not caused by the differences in H-2M (DM) requirement or association with Ii. The time required for processing of specific epitopes appears to be different, suggesting sequential involvement of various endocytic compartments in the antigen processing. Although both WT and MT molecules were found in the early endocytic (transferrin receptor-rich) compartments, MT molecules accumulated in these compartments in higher quantities for longer time periods. Similarly, the MT molecule is retained for a longer time period than WT in late endocytic (LAMP-1 associated) compartments. Together, our data indicate an important role of the TM domain of the MHC class II molecules in the intracellular trafficking and, consequently, antigen processing and presentation.

Transfection

Antigen Presentation - immunology

Blotting, Western

Muramidase - immunology

Microscopy, Confocal

Animals

Histocompatibility Antigens Class II - immunology

Biological Transport

Time Factors

Mice

Epitopes, T-Lymphocyte - immunology

Histocompatibility Antigens Class II - genetics

Cell Compartmentation - immunology

Endocytosis - immunology

Details

Title: Subtitle: Role of the major histocompatibility complex class II transmembrane region in antigen presentation and intracellular trafficking
Creators: Yelena A Barabanova - Department of Microbiology-Immunology, North-western University Medical School, Chicago, IL 60611, USA
Hee-Kap Kang
Jinjong Myoung
Bongsu Kang
Gail A Bishop
Byung S Kim
Resource Type: Journal article
Publication Details: Immunology, Vol.111(2), pp.165-172
Publisher: England
DOI: 10.1111/j.0019-2805.2003.01772.x
PMID: 15027901
PMCID: PMC1782412
ISSN: 0019-2805
eISSN: 1365-2567
Grant note: R01 NS033008 / NINDS NIH HHS NS 33008 / NINDS NIH HHS R01 AI 15546 / NIAID NIH HHS
Language: English
Date published: 02/2004
Academic Unit: Microbiology and Immunology; President
Record Identifier: 9984001152202771

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