Journal article
Role of the nuclease of nontypeable Haemophilus influenzae in dispersal of organisms from biofilms
Infection and immunity, Vol.83(3), pp.950-957
03/2015
DOI: 10.1128/IAI.02601-14
PMCID: PMC4333478
PMID: 25547799
Abstract
Nontypeable Haemophilus influenzae (NTHI) forms biofilms in the middle ear during human infection. The biofilm matrix of NTHI contains extracellular DNA. We show that NTHI possesses a potent nuclease, which is a homolog of the thermonuclease of Staphylococcus aureus. Using a biofilm dispersal assay, studies showed a biofilm dispersal pattern in the parent strain, no evidence of dispersal in the nuclease mutant, and a partial return of dispersion in the complemented mutant. Quantitative PCR of mRNA from biofilms from a 24-h continuous flow system demonstrated a significantly increased expression of the nuclease from planktonic organisms compared to those in the biofilm phase of growth (P < 0.042). Microscopic analysis of biofilms grown in vitro showed that in the nuclease mutant the nucleic acid matrix was increased compared to the wild-type and complemented strains. Organisms were typically found in large aggregates, unlike the wild-type and complement biofilms in which the organisms were evenly dispersed throughout the biofilm. At 48 h, the majority of the organisms in the mutant biofilm were dead. The nuclease mutant formed a biofilm in the chinchilla model of otitis media and demonstrated a propensity to also form similar large aggregates of organisms. These studies indicate that NTHI nuclease is involved in biofilm remodeling and organism dispersal.
Details
- Title: Subtitle
- Role of the nuclease of nontypeable Haemophilus influenzae in dispersal of organisms from biofilms
- Creators
- Christine Cho - University of IowaAroon Chande - University of IowaLokesh Gakhar - University of IowaLauren O Bakaletz - Nationwide Children's HospitalJoseph A Jurcisek - Nationwide Children's HospitalMargaret Ketterer - University of IowaJian Shao - University of IowaKenji Gotoh - University of IowaEric Foster - University of IowaJason Hunt - University of IowaErin O'Brien - University of IowaMichael A Apicella - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.83(3), pp.950-957
- DOI
- 10.1128/IAI.02601-14
- PMID
- 25547799
- PMCID
- PMC4333478
- NLM abbreviation
- Infect Immun
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Grant note
- R01 DC003915 / NIDCD NIH HHS P30 ES005605 / NIEHS NIH HHS AI024616 / NIAID NIH HHS R01 AI024616 / NIAID NIH HHS Howard Hughes Medical Institute R37 AI024616 / NIAID NIH HHS T32 AI007343 / NIAID NIH HHS R56 AI024616 / NIAID NIH HHS R55 DC003915 / NIDCD NIH HHS DC003915 / NIDCD NIH HHS
- Language
- English
- Date published
- 03/2015
- Academic Unit
- Microbiology and Immunology; Biostatistics; Medicine Administration; Internal Medicine
- Record Identifier
- 9984363572202771
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