Role of the transferrin receptor in lymphocyte growth: a rat IgG monoclonal antibody against the murine transferrin receptor produces highly selective inhibition of T and B cell activation protocols

John D Kemp; John A Thorson; Tim H McAlmont; Mark Horowitz; John S Cowdery; Zuhair K Ballas

doi:10.4049/jimmunol.138.8.2422

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Role of the transferrin receptor in lymphocyte growth: a rat IgG monoclonal antibody against the murine transferrin receptor produces highly selective inhibition of T and B cell activation protocols

Journal article

Peer reviewed

Role of the transferrin receptor in lymphocyte growth: a rat IgG monoclonal antibody against the murine transferrin receptor produces highly selective inhibition of T and B cell activation protocols

John D Kemp, John A Thorson, Tim H McAlmont, Mark Horowitz, John S Cowdery and Zuhair K Ballas

The Journal of immunology (1950), Vol.138(8), pp.2422-2426

1987

DOI: 10.4049/jimmunol.138.8.2422

PMID: 2951439

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Abstract

We have produced a new rat IgG monoclonal antibody against the murine transferrin receptor (TR). This antibody (C2F2) exhibits a surprisingly selective pattern of inhibition of murine lymphocyte activation protocols. C2F2 inhibits the mixed lymphocyte reaction (MLR) and the generation of cytotoxic T cells. Interestingly, although interleukin 1 (IL 1)-dependent thymocyte co-stimulatory activity is strongly inhibited by C2F2, interleukin 2 (IL 2)-dependent thymocyte co-stimulation is only marginally reduced. IL 2-dependent growth of CTLL cells is also not inhibited by C2F2. These data suggest that IL 1-dependent helper T cell activation is very sensitive to C2F2-mediated inhibition. Studies with phytohemagglutinin, Concanavalin A, and lipopolysaccharide induced activation also indicate that the inhibitory effects of C2F2 are selective, and T cell activation may be more sensitive to inhibition than B cell activation. Although there is little published information about the functional effects of other rat anti-mouse TR antibodies, the available data suggest that the patterns of inhibition produced by anti-TR antibodies may be individually distinct. Anti-TR antibodies may constitute a new set of highly selective probes for the study of lymphocyte activation.

Immunobiology

Fundamental and applied biological sciences. Psychology

Fundamental immunology

Biological and medical sciences

Organs and cells involved in the immune response

Analysis of the immune response. Humoral and cellular immunity

Details

Title: Subtitle: Role of the transferrin receptor in lymphocyte growth: a rat IgG monoclonal antibody against the murine transferrin receptor produces highly selective inhibition of T and B cell activation protocols
Creators: John D Kemp - Univ. Iowa coll. medicine, dep. pathology, Iowa City IA 52242, United States
John A Thorson - Univ. Iowa coll. medicine, dep. pathology, Iowa City IA 52242, United States
Tim H McAlmont - Univ. Iowa coll. medicine, dep. pathology, Iowa City IA 52242, United States
Mark Horowitz - Univ. Iowa coll. medicine, dep. pathology, Iowa City IA 52242, United States
John S Cowdery - Univ. Iowa coll. medicine, dep. pathology, Iowa City IA 52242, United States
Zuhair K Ballas - Univ. Iowa coll. medicine, dep. pathology, Iowa City IA 52242, United States
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.138(8), pp.2422-2426
DOI: 10.4049/jimmunol.138.8.2422
PMID: 2951439
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: American Association of Immunologists
Language: English
Date published: 1987
Academic Unit: Pathology; Immunology; Internal Medicine
Record Identifier: 9984094494702771

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