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Roles for herpes simplex virus type 1 UL34 and US3 proteins in disrupting the nuclear lamina during herpes simplex virus type 1 egress
Journal article   Open access   Peer reviewed

Roles for herpes simplex virus type 1 UL34 and US3 proteins in disrupting the nuclear lamina during herpes simplex virus type 1 egress

Susan L Bjerke and Richard J Roller
Virology (New York, N.Y.), Vol.347(2), pp.261-276
04/10/2006
DOI: 10.1016/j.virol.2005.11.053
PMCID: PMC2993110
PMID: 16427676
url
https://doi.org/10.1016/j.virol.2005.11.053View
Published (Version of record) Open Access

Abstract

Cells infected with wild type HSV-1 showed significant lamin A/C and lamin B rearrangement, while U L 34-null virus-infected cells exhibited few changes in lamin localization, indicating that U L 34 is necessary for lamin disruption. During HSV infection, U S 3 limited the development of disruptions in the lamina, since cells infected with a U S 3-null virus developed large perforations in the lamin layer. U S 3 regulation of lamin disruption does not correlate with the induction of apoptosis. Expression of either U L 34 or U S 3 proteins alone disrupted lamin A/C and lamin B localization. Expression of U L 34 and U S 3 together had little effect on lamin A/C localization, suggesting a regulatory interaction between the two proteins. The data presented in this paper argue for crucial roles for both U L 34 and U S 3 in regulating the state of the nuclear lamina during viral infection.
Lamin disruption HSV-1 Egress US3 UL34

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