Roles of the TGACT repeat sequence in the yeast TRP5 promoter

W Scott Moye; Howard Zalkin

doi:10.1016/S0021-9258(18)61396-1

Back

Roles of the TGACT repeat sequence in the yeast TRP5 promoter

Journal article

Open access

Peer reviewed

Roles of the TGACT repeat sequence in the yeast TRP5 promoter

W Scott Moye and Howard Zalkin

The Journal of biological chemistry, Vol.262(8), pp.3609-3614

1987

DOI: 10.1016/S0021-9258(18)61396-1

PMID: 3546311

Files and links (1)

url

https://doi.org/10.1016/S0021-9258(18)61396-1View

Published (Version of record) Open Access

Abstract

Yeast genes under general amino acid control contain multiple copies of a sequence known as the TGACT repeat in the 5'-flanking DNA. The yeast TRP5 gene contains two copies of the TGACT repeat sequence in its 5'-flanking region. The upstream TGACT repeat of TRP5 is required for normal basal expression as well as derepression by general control. Synthetic oligonucleotides containing a TGACT sequence were inserted into previously constructed TRP5 control region deletion mutants. A synthetic 17-base pairs (bp) oligonucleotide containing a TGACT copy along with flanking nucleotides from HIS3 was able to restore derepression in all deletion mutants tested. The 17-bp oligonucleotide also functioned bidirectionally. Replacements in the upstream control region by synthetic oligonucleotides indicated that sequences other than the TGACT repeat are required for high basal expression. Replacements of the downstream repeat sequence by the 17-bp oligonucleotide suggest its main role in this position is for derepressed expression. High level derepressed expression was found to correlate with the presence of two repeats.

Molecular Genetics

Biological and medical sciences

Fundamental and applied biological sciences. Psychology

Molecular and cellular biology

Transcription. Transcription factor. Splicing. Rna processing

Details

Title: Subtitle: Roles of the TGACT repeat sequence in the yeast TRP5 promoter
Creators: W Scott Moye - Purdue univ., dep. biochemistry, West Lafayette IN 47907, United States
Howard Zalkin - Purdue univ., dep. biochemistry, West Lafayette IN 47907, United States
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.262(8), pp.3609-3614
DOI: 10.1016/S0021-9258(18)61396-1
PMID: 3546311
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: American Society for Biochemistry and Molecular Biology
Language: English
Date published: 1987
Academic Unit: Molecular Physiology and Biophysics; Internal Medicine
Record Identifier: 9984297496502771

Metrics

12 Record Views

9 Times Cited - Web of Science