Rootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila

Jieyan V Chen; Ling-Rong Kao; Swadhin C Jana; Elena Sivan-Loukianova; Susana Mendonça; Oscar A Cabrera; Clemens Cabernard; Priyanka Singh; Daniel F Eberl; Monica Bettencourt-Dias; Timothy L Megraw

doi:10.1083/jcb.201502032

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Rootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila

Journal article

Open access

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Rootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila

Jieyan V Chen, Ling-Rong Kao, Swadhin C Jana, Elena Sivan-Loukianova, Susana Mendonça, Oscar A Cabrera, Clemens Cabernard, Priyanka Singh, Daniel F Eberl, Monica Bettencourt-Dias, …

The Journal of cell biology, Vol.211(2), pp.435-453

10/26/2015

DOI: 10.1083/jcb.201502032

PMCID: PMC4621839

PMID: 26483560

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Abstract

Cilia are essential for cell signaling and sensory perception. In many cell types, a cytoskeletal structure called the ciliary rootlet links the cilium to the cell body. Previous studies indicated that rootlets support the long-term stability of some cilia. Here we report that Drosophila melanogaster Rootletin (Root), the sole orthologue of the mammalian paralogs Rootletin and C-Nap1, assembles into rootlets of diverse lengths among sensory neuron subtypes. Root mutant neurons lack rootlets and have dramatically impaired sensory function, resulting in behavior defects associated with mechanosensation and chemosensation. Root is required for cohesion of basal bodies, but the cilium structure appears normal in Root mutant neurons. We show, however, that normal rootlet assembly requires centrioles. The N terminus of Root contains a conserved domain and is essential for Root function in vivo. Ectopically expressed Root resides at the base of mother centrioles in spermatocytes and localizes asymmetrically to mother centrosomes in neuroblasts, both requiring Bld10, a basal body protein with varied functions.

Protein Structure, Tertiary

Amino Acid Sequence

Cell Line

Actin Cytoskeleton - metabolism

Cytoskeletal Proteins - genetics

Molecular Sequence Data

Drosophila Proteins - metabolism

Mechanotransduction, Cellular - physiology

Drosophila melanogaster - genetics

Cilia - metabolism

Drosophila melanogaster - metabolism

Sequence Alignment

Animals

Sensory Receptor Cells - cytology

Cytoskeletal Proteins - metabolism

Sensory Receptor Cells - metabolism

Centrioles - metabolism

Drosophila Proteins - genetics

Mechanotransduction, Cellular - genetics

Details

Title: Subtitle: Rootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila
Creators: Jieyan V Chen - Florida State University
Ling-Rong Kao - Department of Biomedical Sciences, Florida State University, Tallahassee, FL 32306
Swadhin C Jana - Instituto Gulbenkian de Ciência, 2780-156 Oeiras, Portugal
Elena Sivan-Loukianova - Biology Department, University of Iowa, Iowa City, IA 52242
Susana Mendonça - Instituto Gulbenkian de Ciência, 2780-156 Oeiras, Portugal
Oscar A Cabrera - Florida State University
Clemens Cabernard - Biozentrum, University of Basel, 4056 Basel, Switzerland
Priyanka Singh - University of Basel
Daniel F Eberl - Biology Department, University of Iowa, Iowa City, IA 52242
Monica Bettencourt-Dias - Instituto Gulbenkian de Ciência, 2780-156 Oeiras, Portugal
Timothy L Megraw - Florida State University
Resource Type: Journal article
Publication Details: The Journal of cell biology, Vol.211(2), pp.435-453
DOI: 10.1083/jcb.201502032
PMID: 26483560
PMCID: PMC4621839
NLM abbreviation: J Cell Biol
ISSN: 0021-9525
eISSN: 1540-8140
Publisher: United States
Grant note: R01 GM068758 / NIGMS NIH HHS GM068758 / NIGMS NIH HHS 14-0236 / Worldwide Cancer Research
Language: English
Date published: 10/26/2015
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9983991958302771

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