Rotary culture enhances pre-osteoblast aggregation and mineralization

S R Facer; R S Zaharias; M E Andracki; J Lafoon; S K Hunter; G B Schneider

doi:10.1177/154405910508400611

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Rotary culture enhances pre-osteoblast aggregation and mineralization

Journal article

Peer reviewed

Rotary culture enhances pre-osteoblast aggregation and mineralization

S R Facer, R S Zaharias, M E Andracki, J Lafoon, S K Hunter and G B Schneider

Journal of dental research, Vol.84(6), pp.542-547

06/2005

DOI: 10.1177/154405910508400611

PMID: 15914592

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Abstract

Three-dimensional environments have been shown to enhance cell aggregation and osteoblast differentiation. Thus, we hypothesized that three-dimensional (3D) growth environments would enhance the mineralization rate of human embryonic palatal mesenchymal (HEPM) pre-osteoblasts. The objective of this study was to investigate the potential use of rotary cell culture systems (RCCS) as a means to enhance the osteogenic potential of pre-osteoblast cells. HEPM cells were cultured in a RCCS to create 3D enviroments. Tissue culture plastic (2D) cultures served as our control. 3D environments promoted three-dimensional aggregate formations. Increased calcium and phosphorus deposition was significantly enhanced three- to 18-fold (P < 0.001) in 3D cultures as compared with 2D environments. 3D cultures mineralized in 1 wk as compared with the 2D cultures, which took 4 wks, a decrease in time of nearly 75%. In conclusion, our studies demonstrated that 3D environments enhanced osteoblast cell aggregation and mineralization.

Microscopy, Electron, Scanning

Osteogenesis - physiology

Calcium - metabolism

Coloring Agents

Humans

Osteoblasts - physiology

Electron Probe Microanalysis

Calcification, Physiologic - physiology

Mesoderm - cytology

Cell Culture Techniques - methods

Rotation

Cell Aggregation - physiology

Palate - embryology

Time Factors

Phosphorus - metabolism

Details

Title: Subtitle: Rotary culture enhances pre-osteoblast aggregation and mineralization
Creators: S R Facer - Department of Endodontics, University of Iowa, College of Dentistry, IA 52242, USA
R S Zaharias
M E Andracki
J Lafoon
S K Hunter
G B Schneider
Resource Type: Journal article
Publication Details: Journal of dental research, Vol.84(6), pp.542-547
DOI: 10.1177/154405910508400611
PMID: 15914592
NLM abbreviation: J Dent Res
ISSN: 0022-0345
eISSN: 1544-0591
Publisher: United States
Grant note: R03DE014269 / NIDCR NIH HHS P60DE13076 / NIDCR NIH HHS
Language: English
Date published: 06/2005
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Dentistry Administration; Prosthodontics; Obstetrics and Gynecology
Record Identifier: 9983930516302771

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