Routine procedures for isolation and identification of enterococci and fecal streptococci

L M Knudtson; P A Hartman

doi:10.1128/AEM.58.9.3027-3031.1992

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Routine procedures for isolation and identification of enterococci and fecal streptococci

Journal article

Open access

Peer reviewed

Routine procedures for isolation and identification of enterococci and fecal streptococci

L M Knudtson and P A Hartman

Applied and environmental microbiology, Vol.58(9), pp.3027-3031

09/1992

DOI: 10.1128/AEM.58.9.3027-3031.1992

PMCID: PMC183043

PMID: 1444416

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https://doi.org/10.1128/AEM.58.9.3027-3031.1992View

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Abstract

Over the past 6 years, a revised classification of the streptococci and enterococci, based primarily on molecular techniques such as 16S rRNA sequencing and DNA-DNA hybridization, emerged. However, little attention was placed on routine physiological tests that could be used in food and clinical laboratories to differentiate between species of a new genus, Enterococcus, and fecal Streptococcus spp. The purpose of this study was to devise a convenient and reliable system to identify enterococci and fecal streptococci by using conventional procedures. Fifty-nine strains of 13 Enterococcus spp., including the type strains and many strains used by previous investigators, were characterized by using conventional tube tests, the API Rapid Strep system, and MicroScan Pos ID panels. Results were compared with each other and with previously published results. A comparison of conventional tube tests versus published tube test results yielded 17 discrepancies. Although not all tests were done with each of the three systems, 28 discrepancies between results obtained with the API system and those obtained with conventional tube tests were found. There were 24 discrepancies between results obtained with the MicroScan Pos ID panel and those obtained with conventional tube tests. There were 12 discrepancies between the results with the API Rapid Strep system and those with the MicroScan Pos ID panels. We devised flow charts of key tests that might be used to identify cultures without resorting to nucleic acid analysis and other labor- and equipment-intensive analyses.

Intestinal Diseases - diagnosis

Streptococcus - classification

Enterobacteriaceae Infections - diagnosis

Streptococcal Infections - diagnosis

Streptococcus - isolation & purification

Diagnostic Tests, Routine - methods

Enterococcus - isolation & purification

Reagent Kits, Diagnostic

Intestinal Diseases - microbiology

Enterococcus - classification

Details

Title: Subtitle: Routine procedures for isolation and identification of enterococci and fecal streptococci
Creators: L M Knudtson - Department of Microbiology, Immunology and Preventive Medicine, Iowa State University, Ames 50011
P A Hartman
Resource Type: Journal article
Publication Details: Applied and environmental microbiology, Vol.58(9), pp.3027-3031
DOI: 10.1128/AEM.58.9.3027-3031.1992
PMID: 1444416
PMCID: PMC183043
ISSN: 0099-2240
eISSN: 1098-5336
Language: English
Date published: 09/1992
Academic Unit: Microbiology and Immunology
Record Identifier: 9984083862802771

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