SARS-CoV-2-specific immunoglobulin Y antibodies are protective in infected mice

Sherif A El-Kafrawy; Abby Odle; Aymn T Abbas; Ahmed M Hassan; Umama A Abdel-Dayem; Arooj K Qureshi; Lok-Yin Roy Wong; Jian Zheng; David K Meyerholz; Stanley Perlman; Alimuddin Zumla; Esam I Azhar

doi:10.1371/journal.ppat.1010782

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SARS-CoV-2-specific immunoglobulin Y antibodies are protective in infected mice

Journal article

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SARS-CoV-2-specific immunoglobulin Y antibodies are protective in infected mice

Sherif A El-Kafrawy, Abby Odle, Aymn T Abbas, Ahmed M Hassan, Umama A Abdel-Dayem, Arooj K Qureshi, Lok-Yin Roy Wong, Jian Zheng, David K Meyerholz, Stanley Perlman, …

PLoS pathogens, Vol.18(9), pp.e1010782-e1010782

09/01/2022

DOI: 10.1371/journal.ppat.1010782

PMCID: PMC9484655

PMID: 36121829

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Abstract

Safe, passive immunization methods are required against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its variants. Immunization of chickens with antigen is known to induce specific IgY antibodies concentrated in the egg yolk and has a good safety profile, high yield of IgY per egg, can be topically applied, not requiring parenteral delivery. Our data provide the first evidence of the prophylactic efficacy of Immunoglobulin Y antibodies against SARS-CoV-2 in mice. Lohmann hens were injected with recombinant SARS-CoV-2 RBD protein; IgY-Abs were extracted from the eggs and characterized using SDS-PAGE. Antiviral activity was evaluated using plaque reduction neutralization tests. In additional experiments, IgY-RBD efficacy was examined in mice sensitized to SARS-CoV-2 infection by transduction with Ad5-hACE2 (mild disease) or by using mouse-adapted virus (severe disease). In both cases, prophylactic intranasal administration of IgY-Abs reduced SARS-CoV-2 replication, and reduced morbidity, inflammatory cell infiltration, hemorrhage, and edema in the lungs and increased survival compared to control groups that received non-specific IgY-Abs. These results indicate that further evaluation of IgY-RBD antibodies in humans is warranted.

Details

Title: Subtitle: SARS-CoV-2-specific immunoglobulin Y antibodies are protective in infected mice
Creators: Sherif A El-Kafrawy
Abby Odle
Aymn T Abbas
Ahmed M Hassan
Umama A Abdel-Dayem
Arooj K Qureshi
Lok-Yin Roy Wong
Jian Zheng
David K Meyerholz
Stanley Perlman
Alimuddin Zumla
Esam I Azhar
Resource Type: Journal article
Publication Details: PLoS pathogens, Vol.18(9), pp.e1010782-e1010782
DOI: 10.1371/journal.ppat.1010782
PMID: 36121829
PMCID: PMC9484655
NLM abbreviation: PLoS Pathog
ISSN: 1553-7366
eISSN: 1553-7374
Publisher: Public Library of Science
Grant note: DOI: 10.13039/501100004919, name: King Abdulaziz City for Science and Technology, award: 20-0004; name: National institute of health, award: PO1 AI060699
Language: English
Date published: 09/01/2022
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Pathology; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9984296997102771

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