SCREEN FOR DOMINANT BEHAVIORAL MUTATIONS CAUSED BY GENOMIC INSERTION OF P-ELEMENT TRANSPOSONS IN DROSOPHILA: AN EXAMINATION OF THE INTEGRATION OF VIRAL VECTOR SEQUENCES

LYLE E FOX; DAVID GREEN; ZIYING YAN; JOHN F ENGELHARDT; CHUN-FANG WU

doi:10.1080/01677060701209235

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SCREEN FOR DOMINANT BEHAVIORAL MUTATIONS CAUSED BY GENOMIC INSERTION OF P-ELEMENT TRANSPOSONS IN DROSOPHILA: AN EXAMINATION OF THE INTEGRATION OF VIRAL VECTOR SEQUENCES

Journal article

Open access

Peer reviewed

SCREEN FOR DOMINANT BEHAVIORAL MUTATIONS CAUSED BY GENOMIC INSERTION OF P-ELEMENT TRANSPOSONS IN DROSOPHILA: AN EXAMINATION OF THE INTEGRATION OF VIRAL VECTOR SEQUENCES

LYLE E FOX, DAVID GREEN, ZIYING YAN, JOHN F ENGELHARDT and CHUN-FANG WU

Journal of neurogenetics, Vol.21(1-2), pp.31-43

2007

DOI: 10.1080/01677060701209235

PMCID: PMC2121585

PMID: 17464796

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https://www.ncbi.nlm.nih.gov/pmc/articles/2121585View

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Abstract

Here we report the development of a high-throughput screen to assess dominant mutation rates caused by P-element transposition within the Drosophila genome that is suitable for assessing the undesirable effects of integrating foreign regulatory sequences (viral cargo) into a host genome. Three different behavioral paradigms were used: sensitivity to mechanical stress, response to heat stress, and ability to fly. The results, from our screen of 35,000 flies, indicate that mutations caused by the random insertion of transposons in Drosophila are more effective at disrupting flight than stress sensitivity. This approach was used to ascertain the frequency of deleterious dominant mutations caused by viral vectors utilized in gene therapy.

Stress sensitivity

Mutagenesis

Simian Virus-40 (SV-40)

Gene therapy

Adeno Associated Virus (AAV)

Complex behaviors

Cytomegalovirus (CMV)

Details

Title: Subtitle: SCREEN FOR DOMINANT BEHAVIORAL MUTATIONS CAUSED BY GENOMIC INSERTION OF P-ELEMENT TRANSPOSONS IN DROSOPHILA: AN EXAMINATION OF THE INTEGRATION OF VIRAL VECTOR SEQUENCES
Creators: LYLE E FOX - Department of Biological Sciences, University of Iowa, Iowa City, Iowa
DAVID GREEN - Department of Biological Sciences, University of Iowa, Iowa City, Iowa
ZIYING YAN - Department of Anatomy and Cell Biology, and Center for Gene Therapy of Cystic Fibrosis and other Genetic Diseases, Carver College of Medicine, University of Iowa, Iowa City, Iowa
JOHN F ENGELHARDT - Department of Anatomy and Cell Biology, and Center for Gene Therapy of Cystic Fibrosis and other Genetic Diseases, Carver College of Medicine, University of Iowa, Iowa City, Iowa
CHUN-FANG WU - Department of Biological Sciences and Center for Gene Therapy of Cystic Fibrosis and other Genetic Diseases, Carver College of Medicine, University of Iowa, Iowa City, Iowa
Resource Type: Journal article
Publication Details: Journal of neurogenetics, Vol.21(1-2), pp.31-43
DOI: 10.1080/01677060701209235
PMID: 17464796
PMCID: PMC2121585
NLM abbreviation: J Neurogenet
ISSN: 0167-7063
eISSN: 1563-5260
Language: English
Date published: 2007
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Iowa Neuroscience Institute; Biology; Radiation Oncology; Internal Medicine
Record Identifier: 9984025450602771

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