Journal article
SK2 channels regulate mitochondrial respiration and mitochondrial Ca2+ uptake
Cell death and differentiation, Vol.24(5), pp.761-773
05/2017
DOI: 10.1038/cdd.2017.2
PMCID: PMC5423111
PMID: 28282037
Abstract
Mitochondrial calcium ([Ca
2+
]
m
) overload and changes in mitochondrial metabolism are key players in neuronal death. Small conductance calcium-activated potassium (SK) channels provide protection in different paradigms of neuronal cell death. Recently, SK channels were identified at the inner mitochondrial membrane, however, their particular role in the observed neuroprotection remains unclear. Here, we show a potential neuroprotective mechanism that involves attenuation of [Ca
2+
]
m
uptake upon SK channel activation as detected by time lapse mitochondrial Ca
2+
measurements with the Ca
2+
-binding mitochondria-targeted aequorin and FRET-based [Ca
2+
]
m
probes. High-resolution respirometry revealed a reduction in mitochondrial respiration and complex I activity upon pharmacological activation and overexpression of mitochondrial SK2 channels resulting in reduced mitochondrial ROS formation. Overexpression of mitochondria-targeted SK2 channels enhanced mitochondrial resilience against neuronal death, and this effect was inhibited by overexpression of a mitochondria-targeted dominant-negative SK2 channel. These findings suggest that SK channels provide neuroprotection by reducing [Ca
2+
]
m
uptake and mitochondrial respiration in conditions, where sustained mitochondrial damage determines progressive neuronal death.
Details
- Title: Subtitle
- SK2 channels regulate mitochondrial respiration and mitochondrial Ca2+ uptake
- Creators
- Birgit Honrath - , MarburgLina Matschke - , MarburgTammo Meyer - , GroningenLena Magerhans - , MarburgFabiana Perocchi - , MunichGoutham K Ganjam - , MarburgHans Zischka - , NeuherbergCornelius Krasel - , MarburgAlbert Gerding - , GroningenBarbara M Bakker - , GroningenMoritz Bünemann - , MarburgStefan Strack - , Iowa City, IANiels Decher - , MarburgCarsten Culmsee - , MarburgAmalia M Dolga - , Marburg
- Resource Type
- Journal article
- Publication Details
- Cell death and differentiation, Vol.24(5), pp.761-773
- Publisher
- Nature Publishing Group
- DOI
- 10.1038/cdd.2017.2
- PMID
- 28282037
- PMCID
- PMC5423111
- ISSN
- 1350-9047
- eISSN
- 1476-5403
- Alternative title
- SK channels and mitochondrial calcium regulation
- Language
- English
- Date published
- 05/2017
- Academic Unit
- Pathology; Iowa Neuroscience Institute; Neuroscience and Pharmacology
- Record Identifier
- 9984040020802771
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