SLP-76 expression is restricted to hemopoietic cells of monocyte, granulocyte, and T lymphocyte lineage and is regulated during T cell maturation and activation

James L Clements; Susan E Ross-Barta; Lorraine T Tygrett; Thomas J. Waldschmidt; Gary A Koretzky

doi:10.4049/jimmunol.161.8.3880

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SLP-76 expression is restricted to hemopoietic cells of monocyte, granulocyte, and T lymphocyte lineage and is regulated during T cell maturation and activation

Journal article

Peer reviewed

SLP-76 expression is restricted to hemopoietic cells of monocyte, granulocyte, and T lymphocyte lineage and is regulated during T cell maturation and activation

James L Clements, Susan E Ross-Barta, Lorraine T Tygrett, Thomas J. Waldschmidt and Gary A Koretzky

The Journal of immunology (1950), Vol.161(8), pp.3880-3889

10/15/1998

DOI: 10.4049/jimmunol.161.8.3880

PMID: 9780153

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Abstract

The leukocyte-specific adapter protein SLP-76 is known to augment the transcriptional activity of nuclear factor of activated T cells and AP-1 following TCR ligation. A role for SLP-76 in additional receptor-mediated signaling events is less clear. To define the pattern of SLP-76 expression during murine hemopoiesis, we stained cells isolated from various tissues with a combination of surface markers followed by intracellular staining with a fluorochrome-labeled SLP-76-specific Ab. In the bone marrow, SLP-76 expression is largely restricted to cells of granulocyte and monocyte lineage. Heterogeneous SLP-76 expression is first detected in the CD44+ CD25- subset within the CD3- CD4- CD8- thymocyte population. Interestingly, SLP-76 expression increases as thymocyte maturation progresses within the CD4- CD8- compartment but decreases as cells mature to a CD4+ CD8+ phenotype. SLP-76 expression is then up-regulated following selection and concomitant with maturation to a CD4+ or CD8+ phenotype. In the periphery, SLP-76 is expressed in T lymphocytes with no detectable expression in the B cell compartment. Exposure to the superantigen staphylococcal enterotoxin B augments SLP-76 expression in the reactive T cell subset. Furthermore, in vitro stimulation with TCR-specific Abs augments the existing levels of SLP-76. These data reveal that SLP-76 expression is coordinately regulated with surface expression of a pre-TCR or mature TCR complex during thymocyte development and that TCR ligation elicits signals that result in increased expression of SLP-76.

Phosphoproteins - immunology

Granulocytes - cytology

Phosphoproteins - biosynthesis

Monocytes - cytology

Lymphatic System - cytology

Monocytes - immunology

Cell Differentiation - immunology

Adaptor Proteins, Signal Transducing

Hematopoietic Stem Cells - immunology

Animals

T-Lymphocytes - cytology

Hematopoietic Stem Cells - cytology

Female

T-Lymphocytes - immunology

Lymphatic System - immunology

Mice

Mice, Inbred BALB C

Granulocytes - immunology

Cell Lineage - immunology

Details

Title: Subtitle: SLP-76 expression is restricted to hemopoietic cells of monocyte, granulocyte, and T lymphocyte lineage and is regulated during T cell maturation and activation
Creators: James L Clements - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242, USA
Susan E Ross-Barta
Lorraine T Tygrett
Thomas J. Waldschmidt
Gary A Koretzky
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.161(8), pp.3880-3889
DOI: 10.4049/jimmunol.161.8.3880
PMID: 9780153
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Language: English
Date published: 10/15/1998
Academic Unit: Pathology
Record Identifier: 9984047757402771

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