Journal article
Salmonella Pathogenicity Island 2-Encoded Proteins SseC and SseD Are Essential for Virulence and Are Substrates of the Type III Secretion System
Infection and immunity, Vol.69(2), pp.737-743
02/2001
DOI: 10.1128/IAI.69.2.737-743.2001
PMCID: PMC97946
PMID: 11159962
Abstract
Survival of
Salmonella enterica
serovar Typhimurium within host phagocytic cells is a critical step in establishing systemic infection in mice. Genes within
Salmonella
pathogenicity island 2 (SPI-2) encode a type III secretion system that is required for establishment of systemic infection. Several proteins encoded by SPI-2 have homology to type III secreted proteins from enteropathogenic
Escherichia coli
and
Yersinia
and, based on that homology, are predicted to be secreted through the SPI-2 type III secretion system. We have investigated the roles of two of these proteins, SseC and SseD. We demonstrate here that the SseD protein is required for systemic
Salmonella
infection of the mouse, and we confirmed the virulence requirement for the SseC protein. Experiments were performed, using cellular fractionation and immunoblotting, to identify the subcellular location of the SseC and SseD proteins. Both proteins were found to localize predominantly to the bacterial cell membrane. In addition, our work revealed that SseC and SseD are exposed to the extracellular environment and are loosely associated with the bacterial membrane. Furthermore, localization of SseC and SseD to the bacterial membrane was found to require a functional SPI-2 type III secretion system. Collectively, these results indicate that the SseC and SseD proteins are secreted by the SPI-2 type III secretion system to the bacterial membrane in order to perform their virulence functions.
Details
- Title: Subtitle
- Salmonella Pathogenicity Island 2-Encoded Proteins SseC and SseD Are Essential for Virulence and Are Substrates of the Type III Secretion System
- Creators
- Joanna R Klein - Department of Microbiology, University of Iowa School of Medicine, Iowa City, Iowa 52242-1109Bradley D Jones - Department of Microbiology, University of Iowa School of Medicine, Iowa City, Iowa 52242-1109
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.69(2), pp.737-743
- DOI
- 10.1128/IAI.69.2.737-743.2001
- PMID
- 11159962
- PMCID
- PMC97946
- NLM abbreviation
- Infect Immun
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 02/2001
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984083878502771
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