Salmonella typhimurium induces membrane ruffling by a growth factor-receptor-independent mechanism

Bradley D Jones; Hugh F Paterson; Alan Hall; Stanley Falkow

doi:10.1073/pnas.90.21.10390

Back

Salmonella typhimurium induces membrane ruffling by a growth factor-receptor-independent mechanism

Journal article

Open access

Peer reviewed

Salmonella typhimurium induces membrane ruffling by a growth factor-receptor-independent mechanism

Bradley D Jones, Hugh F Paterson, Alan Hall and Stanley Falkow

Proceedings of the National Academy of Sciences - PNAS, Vol.90(21), pp.10390-10394

11/01/1993

DOI: 10.1073/pnas.90.21.10390

PMCID: PMC47780

PMID: 8234304

Files and links (1)

url

https://doi.org/10.1073/pnas.90.21.10390View

Published (Version of record) Open Access

Abstract

Invasive Salmonella typhimurium induces dramatic actin rearrangements on the membrane surface of mammalian cells as part of its entry mechanism. These changes, which are best characterized as membranous ruffles, closely resemble the membrane changes that occur when a growth factor binds to its receptor. Recently, inhibition of the function of the small GTPases rac and rho in quiescent serum-starved fibroblasts was demonstrated to abolish growth factor-mediated ruffling and stress-fiber formation, respectively. In addition, actin changes induced by the oncogene ras were also shown to be regulated by rac and rho. Because Salmonella-induced actin rearrangements resemble those caused by growth factors, we investigated whether ras, rho, or rac regulates the membrane ruffling elicited by S. typhimurium. Surprisingly, inhibition of the functions of these GTPases had no effect on the ability of invasive S. typhimurium to induce membrane ruffles on a variety of tissue culture cells including Madin-Darby canine kidney cells, Swiss 3T3 fibroblasts, and Hep-2 cells. These results led us to examine the interactions of S. typhimurium with Henle-407 intestinal cells, which lack epidermal growth factor receptor on their membrane surface. We found no difference in the ability of invasive S. typhimurium to induce membrane ruffling and to enter Henle-407 cells with or without the epidermal growth factor receptor on the membrane surface. We, therefore, conclude that invasive S. typhimurium induces membrane ruffling and its own internalization by a rac-independent, growth factor-receptor-independent signaling pathway.

Cell Line

Salmonella typhimurium - physiology

Intestines

Humans

Actins - metabolism

Brefeldin A

Cells, Cultured

Actins - ultrastructure

Cell Membrane - ultrastructure

Cyclopentanes - pharmacology

Kidney

ErbB Receptors - physiology

Animals

GTP Phosphohydrolases - metabolism

Dogs

Cell Membrane - metabolism

Mice

Epidermal Growth Factor - pharmacology

Tumor Cells, Cultured

3T3 Cells

Cell Membrane - drug effects

Details

Title: Subtitle: Salmonella typhimurium induces membrane ruffling by a growth factor-receptor-independent mechanism
Creators: Bradley D Jones - Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305-5402
Hugh F Paterson
Alan Hall
Stanley Falkow
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.90(21), pp.10390-10394
DOI: 10.1073/pnas.90.21.10390
PMID: 8234304
PMCID: PMC47780
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Grant note: AI08404 / NIAID NIH HHS DK38707 / NIDDK NIH HHS AI26195 / NIAID NIH HHS
Language: English
Date published: 11/01/1993
Academic Unit: Microbiology and Immunology
Record Identifier: 9984083236402771

Metrics

23 Record Views

103 Times Cited - Web of Science