Journal article
Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death
Journal of Visualized Experiments, Vol.2017(128), e56535
10/31/2017
DOI: 10.3791/56535
PMCID: PMC5755276
PMID: 29155746
Abstract
Macrophages highly express epidermal fatty acid-binding protein and adipose fatty acid-binding protein. They actively uptake saturated and unsaturated fatty acids, which might play a critical role in regulating their immune functions. Numerous studies have shown that various fatty acids, saturated or unsaturated, may possess different impacts on cell growth and function. However, the approaches used for fatty acid preparation vary, which may lead to non-physiological results. Serum albumin, a natural carrier for fatty acids in mammalian peripheral blood, is recommended for forming a conjugate complex with the sodium salt of fatty acids to study fatty acid function in mammalian cells, thus minimizing the toxicity of fatty acid soap. Thus, a simple, relatively quick heating and sonicating method is developed and presented here for BSA-fatty acid conjugate formation. We describe a protocol using saturated fatty acids, especially stearic acids to induce severe cell death in mouse bone-marrow derived macrophages. We further demonstrate that saturated fatty acid-induced cell death is positively associated with accumulated cellular ceramide levels. This method can be extended for studies of the impact of fatty acid on other mammalian cells.
Details
- Title: Subtitle
- Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death
- Creators
- Yuwen Zhang - University of LouisvilleJiaqing Hao - University of LouisvilleYanwen Sun - University of LouisvilleBing Li - University of Louisville
- Resource Type
- Journal article
- Publication Details
- Journal of Visualized Experiments, Vol.2017(128), e56535
- Publisher
- MyJove Corporation
- DOI
- 10.3791/56535
- PMID
- 29155746
- PMCID
- PMC5755276
- ISSN
- 1940-087X
- eISSN
- 1940-087X
- Language
- English
- Date published
- 10/31/2017
- Academic Unit
- Pathology; Surgery
- Record Identifier
- 9984695788302771
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