Journal article
Scale-up of pseudo solid-phase enzymatic synthesis of alpha-methyl glucoside acrylate
Biotechnology and bioengineering, Vol.79(1), pp.15-22
07/05/2002
DOI: 10.1002/bit.10272
PMID: 17590927
Abstract
The successful scale-up of the enzymatic synthesis of alpha-methyl glucoside acrylate from laboratory-scale (milliliter) to pilot-scale (liter) was examined. Specifically, Candida antarctica lipase B (Novozym 435) was used as a biocatalyst to produce alpha-methyl glucoside acrylate via the transesterification of alpha-methyl glucoside (MG) with vinyl acrylate (VA) using acetone as a solvent. This is a pseudo-solid-phase synthesis; only a fraction of the alpha-methyl glucoside and the product are soluble in acetone. Molecular sieves were used to remove traces of water in the reaction medium and to increase enzyme stability by removing the acetaldehyde by-product. A general method was also developed to purify and recover the monoacrylate product from unreacted sugar and undesired diester by a simple crystallization and precipitation process.
Details
- Title: Subtitle
- Scale-up of pseudo solid-phase enzymatic synthesis of alpha-methyl glucoside acrylate
- Creators
- Yanzi Li - Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City, Iowa 52242, USADavid G Rethwisch
- Resource Type
- Journal article
- Publication Details
- Biotechnology and bioengineering, Vol.79(1), pp.15-22
- Publisher
- United States
- DOI
- 10.1002/bit.10272
- PMID
- 17590927
- ISSN
- 0006-3592
- eISSN
- 1097-0290
- Language
- English
- Date published
- 07/05/2002
- Academic Unit
- Chemical and Biochemical Engineering
- Record Identifier
- 9984003483102771
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