Journal article
Scaleup of Insect Cell Cultures: Protective Effects of Pluronic F-68
Nature biotechnology, Vol.6(12), pp.1411-1418
12/1988
DOI: 10.1038/nbt1288-1411
Abstract
We demonstrate that insect cells can be grown in an agitated, sparged bioreactor and an airlift bioreactor with a growth rate and maximum cell density comparable to those obtained in spinner flasks oxygenated by surface diffusion. Recombinant protein synthesis by cells infected with a genetically-modified baculovirus in the sparged bioreactor is also demonstrated. The nonionic surfactant Pluronic® F-68 was responsible for the protection of insect cells from the adverse effects of sparging. The mechanism of Pluronic® F-68 protection in a sparged environment is discussed.
Details
- Title: Subtitle
- Scaleup of Insect Cell Cultures: Protective Effects of Pluronic F-68
- Creators
- David W MurhammerCharles F Goochee
- Resource Type
- Journal article
- Publication Details
- Nature biotechnology, Vol.6(12), pp.1411-1418
- DOI
- 10.1038/nbt1288-1411
- ISSN
- 1087-0156
- eISSN
- 1546-1696
- Language
- English
- Date published
- 12/1988
- Academic Unit
- Chemical and Biochemical Engineering
- Record Identifier
- 9984003987302771
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