Journal article
Scanning Thin-Sheet Laser Imaging Microscopy Elucidates Details on Mouse Ear Development
Developmental dynamics, Vol.241(3), pp.465-480
03/2012
DOI: 10.1002/dvdy.23736
PMCID: PMC5010664
PMID: 22271591
Abstract
Background: The mammalian inner ear is transformed from a flat placode into a three-dimensional (3D) structure with six sensory epithelia that allow for the perception of sound and both linear and angular acceleration. While hearing and balance problems are typically considered to be adult onset diseases, they may arise as a developmental perturbation to the developing ear. Future prevention of hearing or balance loss requires an understanding of how closely genetic mutations in model organisms reflect the human case, necessitating an objective multidimensional comparison of mouse ears with human ears that have comparable mutations in the same gene.
Results: Here, we present improved 3D analyses of normal murine ears during embryonic development using optical sections obtained through Thin-Sheet Laser Imaging Microscopy. We chronicle the transformation of an undifferentiated otic vesicle between mouse embryonic day 11.5 to a fully differentiated inner ear at postnatal day 15.
Conclusions: Our analysis of ear development provides new insights into ear development, enables unique perspectives into the complex development of the ear, and allows for the first full quantification of volumetric and linear aspects of ear growth. Our data provide the framework for future analysis of mutant phenotypes that are currently under-appreciated using only two dimensional renderings.
Details
- Title: Subtitle
- Scanning Thin-Sheet Laser Imaging Microscopy Elucidates Details on Mouse Ear Development
- Creators
- Benjamin Kopecky - Department of Biology, University of Iowa, Iowa City, IowaShane Johnson - Department of Otolaryngology, University of Minnesota, Minneapolis, MinnesotaHeather Schmitz - Department of Otolaryngology, University of Minnesota, Minneapolis, MinnesotaPeter Santi - Department of Otolaryngology, University of Minnesota, Minneapolis, MinnesotaBernd Fritzsch - Department of Biology, University of Iowa, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Developmental dynamics, Vol.241(3), pp.465-480
- DOI
- 10.1002/dvdy.23736
- PMID
- 22271591
- PMCID
- PMC5010664
- NLM abbreviation
- Dev Dyn
- ISSN
- 1058-8388
- eISSN
- 1097-0177
- Language
- English
- Date published
- 03/2012
- Academic Unit
- Psychological and Brain Sciences; Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984070529602771
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