Journal article
Scattering and absorption effects in the determination of glucose in whole blood by near-infrared spectroscopy
Analytical chemistry (Washington), Vol.77(14), pp.4587-4594
2005
DOI: 10.1021/ac0504161
PMID: 16013877
Abstract
Optical properties of whole bovine blood are examined under conditions of different glucose loadings. A strong dependency is established between the scattering properties of the whole blood matrix and the concentration of glucose. This dependency is explained in terms of variations in the refractive index mismatch between the scattering bodies (predominately red blood cells) and the surrounding plasma. Measurements in the presence of a well-known glucose transport inhibitor indicate that variations in refractive index mismatch are related to the penetration of glucose into the red blood cells and demonstrate that increased scattering involves the uptake of glucose by red blood cells. Finally, multivariate calibration models are presented for the measurement of glucose in a whole blood matrix. These models are based on near-infrared spectral data collected from 80 different samples prepared from a single whole blood matrix. Calibration studies are performed over the combination, first-over-tone, and short-wavelength spectral regions. The best calibration model is generated from combination region spectra, providing a standard error of prediction (SEP) of less than 1 mM over the concentration range of 3-30 mM. The model based on the first-overtone region is slightly degraded but still provides acceptable performance (SEP = 1.20 mM). The model based on the short-wavelength region is further degraded (SEP = 2.53 mM). To rationalize these results, an analysis of the selectivity of the calibration models is performed by computing the glucose net analyte signal. It is established that the models based on the combination and first-overtone regions are dominated by glucose absorption information, while the model computed from the short-wavelength region is based primarily on scattering information. This result provides evidence that absorption information is needed in order to obtain a glucose calibration model with acceptable performance. © 2005 American Chemical Society.
Details
- Title: Subtitle
- Scattering and absorption effects in the determination of glucose in whole blood by near-infrared spectroscopy
- Creators
- Airat K Amerov - Department of Chemistry & Optical Science and Technology Center, University of Iowa, Iowa City, Iowa 52242, United StatesJUN Chen - Department of Chemistry & Optical Science and Technology Center, University of Iowa, Iowa City, Iowa 52242, United StatesGary W Small - Department of Chemistry & Optical Science and Technology Center, University of Iowa, Iowa City, Iowa 52242, United StatesMark A Arnold - Department of Chemistry & Optical Science and Technology Center, University of Iowa, Iowa City, Iowa 52242, United States
- Resource Type
- Journal article
- Publication Details
- Analytical chemistry (Washington), Vol.77(14), pp.4587-4594
- Publisher
- American Chemical Society
- DOI
- 10.1021/ac0504161
- PMID
- 16013877
- ISSN
- 0003-2700
- eISSN
- 1520-6882
- Language
- English
- Date published
- 2005
- Academic Unit
- Center for Biocatalysis and Bioprocessing; Fraternal Order of Eagles Diabetes Research Center; Chemistry
- Record Identifier
- 9984216682902771
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