Journal article
Schwann cell‐conditioned medium supports neurite outgrowth and survival of spinal cord neurons in culture
Muscle & nerve, Vol.11(4), pp.324-330
04/1988
DOI: 10.1002/mus.880110408
PMID: 3041275
Abstract
The effect of Schwann cell‐conditioned medium (SCM) on the development in vitro of spinal cord neurons was studied. Spinal cord neurons from 18‐day‐old rat embryos were cultured in serum‐free conditioned medium obtained from confluent rat Schwann cells. In cultures fed SCM, the cells developed typical neuronal morphology and were identified by indirect immunofluorescence using a monoclonal antibody to neurofilament protein. SCM stimulated neurite outgrowth and supported survival of spinal cord neurons. Preliminary characterization suggests that the neurotrophic factor in SCM appears to be a protein with a molecular weight greater than 8000 daltons.
Details
- Title: Subtitle
- Schwann cell‐conditioned medium supports neurite outgrowth and survival of spinal cord neurons in culture
- Creators
- E. Peter BoschJose G AssoulineNicholas J PantazisRamon Lim Lim
- Resource Type
- Journal article
- Publication Details
- Muscle & nerve, Vol.11(4), pp.324-330
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- DOI
- 10.1002/mus.880110408
- PMID
- 3041275
- ISSN
- 0148-639X
- eISSN
- 1097-4598
- Number of pages
- 7
- Language
- English
- Date published
- 04/1988
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Neurology; Anatomy and Cell Biology; Iowa Neuroscience Institute; Urology
- Record Identifier
- 9984065476602771
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