Screening of a large global Aspergillus fumigatus species complex collection by using a species-specific microsphere-based Luminex assay

Kizee A Etienne; Lalitha Gade; Shawn R Lockhart; Daniel J Diekema; Shawn A Messer; Michael A Pfaller; S Arunmozhi Balajee

doi:10.1128/JCM.01415-09

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Screening of a large global Aspergillus fumigatus species complex collection by using a species-specific microsphere-based Luminex assay

Journal article

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Peer reviewed

Screening of a large global Aspergillus fumigatus species complex collection by using a species-specific microsphere-based Luminex assay

Kizee A Etienne, Lalitha Gade, Shawn R Lockhart, Daniel J Diekema, Shawn A Messer, Michael A Pfaller and S Arunmozhi Balajee

Journal of clinical microbiology, Vol.47(12), pp.4171-4172

12/2009

DOI: 10.1128/JCM.01415-09

PMCID: PMC2786642

PMID: 19794043

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Abstract

A microsphere-based Luminex assay was developed and validated for rapid identification of Aspergillus fumigatus from the other species within the A. fumigatus species complex (section Fumigati). This molecular tool was then employed to screen 499 clinical A. fumigatus species complex isolates collected from multiple medical centers throughout the world with results demonstrating the exclusive presence of A. fumigatus.

Aspergillus fumigatus - classification

Molecular Diagnostic Techniques - methods

DNA, Fungal - analysis

DNA, Fungal - genetics

Species Specificity

Humans

Aspergillosis - diagnosis

Aspergillus fumigatus - genetics

Microspheres

Aspergillus fumigatus - isolation & purification

Sensitivity and Specificity

Aspergillosis - microbiology

DNA Probes - genetics

Details

Title: Subtitle: Screening of a large global Aspergillus fumigatus species complex collection by using a species-specific microsphere-based Luminex assay
Creators: Kizee A Etienne - Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
Lalitha Gade
Shawn R Lockhart
Daniel J Diekema
Shawn A Messer
Michael A Pfaller
S Arunmozhi Balajee
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.47(12), pp.4171-4172
DOI: 10.1128/JCM.01415-09
PMID: 19794043
PMCID: PMC2786642
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Publisher: United States
Language: English
Date published: 12/2009
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Internal Medicine
Record Identifier: 9983986369702771

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